镉对大鼠输精管损伤及锌保护的超微结构与葡萄糖-6-磷酸酶细胞化学的研究

目的研究小剂量氯化镉对大鼠输精管近、远段的影响及锌的保护作用。方法用１％氯化镉（２ｍｇ／ｋｇ体重）腹腔注射雄性Ｗｉｓｔａｒ大鼠后４ｈ到６０ｄ及镉、锌联合注射后３ｄ、１５ｄ取材。采用电镜观察和葡萄糖－６－磷酸酶（Ｇ－６－Ｐａｓｅ）电镜细胞化学定量研究。结果镉注射４ｈ后，输精管主细胞出现超微结构改变，３～７ｄ改变最明显，１５ｄ后有所减轻，６０ｄ后基本恢复正常。主细胞的主要改变为线粒体肿胀，内质网扩张及髓样结构增多。镉注射后４ｈ，远段主细胞Ｇ－６－Ｐａｓｅ反应产物明显减少，３ｄ最少，１５ｄ反应产物增多。镉、锌联合注射后超微结构及Ｇ－６－Ｐａｓｅ活性损伤均较单纯镉组轻。结论锌对镉所致大鼠输精管主细胞超微结构损伤及远段主细胞Ｇ－６－Ｐａｓｅ活性影响有明显保护作用。

THE INJURIOUS EFFECTS OF CADMIUM AND PROTECTION OF ZINC ON ULTRASTRUCTURE AND CYTOCHEMISTRY OF G-6-Pase OF VAS DEFERENS IN RATS

Objective To observe the epithelial types and ultrastructural features of the human nasopharynx and to investigate the cellular origin and histological classification of nasopharyngeal carcinoma. Methods The adult nasopharyngeal mucosa of sixty two cases, which were taken from the top or lateral wall of the nasopharynx, were observed with light, and electron microscopes. Results Pseudostratified ciliated columnar epithelium was predominant type in the regions, where nasopharyngeal carcinoma often occurs, squamous epithelium was secondary, and the latter could be derived from the former through metaplasia. The basal cells of epithelia in the nasopharynx had similar ultrastructural features. Conclusion Under certain carcinogenic stimulation, these basal cells can transform into either keratinized or non keratinized carcinoma(poorly differentiated squamous cell carcinoma and undifferentiated carcinoma), occationally adenocarcinoma.