利拉鲁肽对大鼠压力负荷型慢性心衰的保护作用

目的研究利拉鲁肽对压力负荷型慢性心衰大鼠心脏功能的作用及其机制。方法 30只SD大鼠随机分成假手术组、心衰组、利拉鲁肽组,每组10只大鼠。心衰组、利拉鲁肽组采用腹主动脉缩窄法制备模型,假手术组只穿线不缩窄。利拉鲁肽组术后12周给予利拉鲁肽皮下注射,2 mg/kg·d,共30 d。假手术组和心衰组给予同等剂量的生理盐水。给药30 d后给大鼠称重,并使用压力容积系统来检测血流动力学指标,同时对大鼠的各个器官称重。将大鼠麻醉后,腹主动脉抽血离心后取血清,按试剂盒方法测定超氧化物歧化酶(SOD)、B型钠尿肽(BNP)、丙二醛(MDA)。结果心脏称重显示,利拉鲁肽组的心脏重量明显低于心衰组(P0.05)。压力容积系统检测血流动力学显示,相比于假手术组,利拉鲁肽组大鼠的Ves、Ved、Pmax、Pes、Ped值均有明显降低(P0.05),d P/dt max、-d P/dt min、EF和Pow Max值则明显增加(P0.05)。和心衰组相比,利拉鲁肽组大鼠血清中的SOD值明显增加(P0.05),BNP值则明显降低(P0.05)。结论利拉鲁肽可以改善心衰大鼠左心室的收缩和舒张功能,减轻心肌细胞的损伤,其机制可能与抗氧化作用相关。

Liraglutide protects pressure-overload rat heart from failure

Objective To explore the effect of liraglutide on pressure-overload chronic heart failure in rats and related mechanisms. Methods Totally 30 SD rats were randomly divided into sham operation group, heart failure group and liraglutide group.The animals were anaesthetized and a Millar pressure volume conductance catheter SPR-838) was inserted through right carotid artery into LV to measure pressure-volume (P-V) loop.Body and organ weight were measured. After the end of intervention, the rats were anesthetized, and abdominal aortic blood taken from the upper serum after centrifugation. Kit method was used to measure superoxide dismutase (SOD), brain natriuretic peptide (BNP), malondialdehyde (MDA).Results Compared with those of the sham operation group, there was a increase in absolute heart weight and (P<0.05). During the P-V loop analyses, We found that left ventricular (LV) end-systolic volume, end-diastolic volume, end-systolic pressure, end-diastolic pressure and maximum pressure were all remarkablely lower in liraglutide group than HF group in rats (P<0.05). Peak rate of pressure rise (dP/dt max), peak rate of pressure decline (-dP/dt min), ejection fraction and Maximum Power were increased in liraglutide group comparable by HF group (P<0.05). SOD activity was significantly increased and BNP concentration was significantly decreased in liraglutide group compared to HF group (P<0.05). Conclusions These results show that liraglutide protects pressure-overload rat heart from failure possibly through reducing oxidation.