基于UPLC-Q-TOF-MSE分析芍枣胶囊在大鼠体内的代谢产物

目的:研究芍枣胶囊在抑郁模型大鼠体内的代谢产物,并归纳和总结其代谢途径。方法:大鼠灌胃芍枣胶囊提取液后,收集血液、尿液、粪便和全脑并进行预处理,采用UPLC-Q-TOF-MS~E分析,流动相0.1%甲酸-乙腈梯度洗脱,流速0.6m L·min~(-1),柱温45℃,在负离子模式下获得MS/MS~E质谱信息,通过Metabolynx软件处理后对芍枣胶囊中活性化合物在血浆、尿液、粪便和全脑中的代谢产物进行推测。结果:大鼠体内共鉴定得到代谢产物7个,其中芍药苷的2个,斯皮诺素的3个,3,6'-二芥子酰基蔗糖的2个。结论:芍枣胶囊中活性化合物芍药苷,斯皮诺素和3,6'-二芥子酰基蔗糖主要代谢产物存在于血液、尿液和粪便中,代谢途径主要有脱糖水解、甲基化、脱羰基等。

Analysis on in Vivo Metabolites of Shaozao Capsules in Rats by UPLC-Q-TOF-MSE

Objective: To analyze three bioactive compounds from Shaozao capsules and their metabolites in depression model rats,and summarize their metabolic pathways. Method: After oral administration of Shaozao capsules extract,rat plasma,urine,feces and brain were collected,these samples were analyzed by UPLC-Q-TOF-MS^E,mobile phase was acetonitrile-0.1% formic acid for gradient elution,flow rate was 0.6 mL·min^-1,metabolites of bioactive compounds were processed with Metabolynx software. Result: According to the mass information,7 metabolites were discovered,including 3 metabolites of peoniflorin,2 metabolites of spinosin and 2 metabolites of 3, 6''-disinapoyl sucrose. Conclusion: The metabolites mainly exist in rat plasma,urine and feces.The metabolic reactions of these active compounds form Shaozao capsules are methylation,decarbonylation and hydrolysis-desugar,et al.