成人牙周炎患者全唾液蛋白质凝胶电泳分析

目的　筛选与成人牙周炎(AP)相关的唾液蛋白因子。方法　分为AP组和正常对照(NC组)两组,每组25例,收集非刺激性全唾液,采用十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)法分离唾液蛋白并分析其成分。AP组牙周基础治疗后2周、4周重复采样与分析。结果　AP组治疗前唾液中66 kD、18 kD、15 kD、13 kD蛋白条带容量值,51 kD、34 kD、19 kD、17 kD蛋白条带出现率都显著高于NC组;牙周基础治疗后以上各变量都显著下降。结论　18 kD、15 kD、13 kD与AP的发生与转归有关;牙周炎症状态下唾液中血清性蛋白增加。

Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis of Whole Salivary Proteins from Patients with Adult Periodontitis

Objective\ This study was intended to determine the salivary protein factors related to adult periodontitis.Methods\ Twenty_five patients with adult periodontitis (AP group) and twenty_five normal controls (NC group, 25) were adopted in the study. Salivary protein composition in unstimulated whole saliva samples obtained before and after basic treatment was analyzed by SDS_PAGE.Results\ The volume of proteins with 66 kD, 18 kD, 15 kD, 13 kD in AP group before treatment were all remarkably higher than those in NC group. In addition, 51 kD, 34 kD, 19 kD, 17 kD protein bands were more frequently observed in AP group by comparison with NC group. However, after initial therapy, all the variables mentioned above decreased remarkably.Conclusion\ The results demonstrated that protein bands with 18 kD, 15 kD, 13 kD might be closely related to the occurrence and recovery of periodontitis, and serum_derived proteins in saliva increased in patients with adult periodontitis.