| 中耳胆脂瘤上皮细胞DNA的特征 |
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| 引用本文： | 崔永华,潘宏光,高起学.中耳胆脂瘤上皮细胞DNA的特征[J].临床耳鼻咽喉头颈外科杂志,2001,15(2):62-63. |
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| 作者姓名： | 崔永华 潘宏光 高起学 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院耳鼻咽喉科( |
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| 摘 要： | 目的 :采用流式细胞术分析胆脂瘤上皮细胞的 DNA特征 ,探讨胆脂瘤的病理学属性。方法 :利用机械捣碎法将新鲜的胆脂瘤和外耳道上皮制成单细胞悬液 ;采用 PI法 ,对 2 5例胆脂瘤上皮和 10例正常外耳道细胞上皮的 DNA进行流式细胞术分析。结果 :胆脂瘤上皮细胞和外耳道上皮细胞的核型均为二倍体 ,未见异倍体。胆脂瘤上皮细胞 S期细胞数 (10 .9± 2 .32 ) %明显高于外耳道上皮 (7.31± 2 .82 ) %。结论 :虽然胆脂瘤破坏力强 ,易复发 ,但从染色体水平看来 ,仍提示为一种炎性病变 ,未见有恶变倾向。
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| 关 键 词： | 中耳胆脂瘤 脱氧核糖核酸 上皮细胞 流式细胞术 |
| 文章编号： | 1001-1781(2001)02-0062-02 |
| 修稿时间： | 2000年6月21日 |
DNA character of the cholesteatoma |
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| CUI Yonghua,Pan Hongguang,GAO Qixue.DNA character of the cholesteatoma[J].Journal of Clinical Otorhinolaryngology,2001,15(2):62-63. |
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| Authors: | CUI Yonghua Pan Hongguang GAO Qixue |
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| Institution: | Department of Otolaryngology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030. |
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| Abstract: | OBJECTIVE: To compare the DNA character with in cholesteatoma tissues with that in normal skin. METHOD: DNA content within 25 cholesteatoma tissues and 10 normal postauricular skin was measured with flow cytometry. RESULT: The content of cholesteatoma tissues and postauricular skin specimens are a normal euploid. The cell number during S phase of cholesteatoma (10.9 +/- 2.32)% was higher than that in normal skin(7.31 +/- 2.82)%. CONCLUSION: Although the cholesteatoma has a very high proliferating character, the result suggest it isn' t a malignant tumor. |
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| Keywords: | Middle ear Sholesteatoma DNA Flow Cytometry Epithelium |
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