环磷酰胺对大鼠睾丸和附睾的影响

目的探讨抗肿瘤药物所致大鼠睾丸和附睾损害,为寻找男性不育症中药治疗的研究提供理论依据。方法选用16只15周龄SD大鼠随机分为对照组和实验组,每组8只;实验组腹腔注射环磷酰胺20mg·kg-1·d-1,连续5d,用药2个月后,应用HE染色法研究大鼠睾丸、附睾远期组织学变化,用原位缺口末端标记法(TUNEL方法)检测生精细胞凋亡。结果实验组大鼠体重、睾丸和附睾重量均显著减轻(P<0.01),睾丸生精小管直径缩小、间距增宽、生精上皮变薄、生精细胞层次和数量减少、生精小管腔多未见精子形成,实验组睾丸生精小管直径、面积、生精上皮细胞数、均显著低于对照组(P<0.01);实验组生精细胞凋亡增多,与对照组比较,生精细胞显著凋亡(P<0.01);附睾管管腔内腔内精子稀少,含有大量脱落细胞,管壁变薄。结论环磷酰胺对大鼠睾丸、附睾远期损害明显,促进生精细胞凋亡。

The effect of cyclophosphamide on the testis and epididymis of rat

Objective To investigate the toxic effect of cyclophosphamide (CP) on the testis and epididymis of rat. Methods Sixteen 15 weeks male SD rats were randomly divided into control and experimental group,. The rats of experimental group were injected intraperitoneally with cyclophosphamide of 20 mg·kg -1·d -1. After two months of administration of cyclophosphamide, the histological changes were examined by light microscopy, the spermatogenic cell apoptosis was visualized by TUNEL. Results The weight of testis and epididymis was significantly reduced (P<0.01), the average diameter and area of seminiferous tubule and cell number of spermatogenic epithelium in experimental groups were lower than those in controls (P< 0.01). the spermatozoon was not found in the seminiferous tubule and epididymal duct, the apoptosis was significantly increased (P<0.01). Conclusion After two months of administration of cyclophosphamide, the toxic effect of cyclophosphamide on testis and epididymis of rat is obvious, the spermatogenic cell apoptosis was significantly increased.