野生型p53基因抑制舌鳞癌细胞株Tca8113在裸鼠体内生长及增殖研究

为考察野生型ｐ５３基因转移入舌鳞癌细胞株Ｔｃａ８１１３后，癌株在裸鼠体内的生长及增殖状态，作者采用电穿孔方法分４组进行了如下基因转移：野生型ｐ５３基因，空白对照质粒，突变型ｐ５３基因和未转移任何基因的空白对照。在基因转移进入舌鳞癌细胞株Ｔｃａ８１１３后，分别接种于１６只裸鼠体内。成瘤后分别进行常规病理学、定量病理学和免疫组织化学研究。结果：转染野生型ｐ５３基因组与其余３组比较，其形成的肿瘤重量明显为轻；肿瘤异常核分裂相减少；定量病理学显示肿瘤坏死区面积所占瘤体总面积的百分比明显降低；免疫组织化学显示非坏死区增殖细胞核抗原明显更低。上述结果提示肿瘤细胞获取了功能完好的外源性野生型ｐ５３基因后，其基因表达并产生了新的负性调节因子，抑制了肿瘤的生长和增殖。并从理论上证明了利用野生型ｐ５３基因口腔粘膜鳞癌进行基因治疗是可能的。

The Inhibitory Effect of Wild type p53 Gene on the Growth and Proliferation of Lingual Squamous Cell Carcioma Cell Line Tca8113 in Nude Mice *

The aim of this study was to observe the growth and proliferation of the Tca8113 cell line in nude mice after the wild type p53 gene has been transfered by electroporation. The experiment consisted of four groups: the wild type p53 gene, blank control plasmid, the mutant p53 gene, and blank control into which no gene was transfered. After the gene was transfected, the cells were planted into 16 nude mice respectively. When the tumor formed, the nude mice were killed and the tumors were studied by regular pathology, stereology and immunohistochemistry; The results showed that in the wild type p53 gene group, the tumors, the wild type p53 gene group was compared.The results showed that in the wild type p53 gene group, the tumors weighed less heavy, the pathological mitosis decreased markedly, and stereologically, the ratio of the area of necrosis to the total tumorous area was smaller. Similarly, the count of the proliferation cell nuclear antigen in non necrotic area went down. The above findings were statistically significant ( P <0 05).These indicate that when the tumor cells re obtain the well functional exogenic wild type p53 gene, the gene expresses and produces new negative cell growth regulator to replace the abnormal regulation system partially, then the growth and proliferation of the tumors can be inhibited; therefore, it is suggested that the p53 tumor suppressor gene may possibly be used as a gene therapy agent to treat oral squamous cell carcinoma.