| 野西瓜总生物碱诱导HepG2细胞凋亡与[Ca2+]i变化的相关性 |
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| 引用本文： | 于蕾,莫科,王崴,崔荣田,邹翔,季宇彬.野西瓜总生物碱诱导HepG2细胞凋亡与[Ca2+]i变化的相关性[J].中草药,2009,40(2):255-258. |
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| 作者姓名： | 于蕾 莫科 王崴 崔荣田 邹翔 季宇彬 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100102;哈尔滨商业大学生命科学与环境科学研究中心,黑龙江,哈尔滨,150076;抗肿瘤天然药物教育部工程研究中心,黑龙江,哈尔滨,150076
2. 哈尔滨商业大学生命科学与环境科学研究中心,黑龙江,哈尔滨,150076;抗肿瘤天然药物教育部工程研究中心,黑龙江,哈尔滨,150076 |
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| 摘 要： | 目的 探讨野西瓜总生物碱(Capparis spinosa alkaloid,CSA)对人肝癌HepG2细胞的杀伤和诱导凋亡的作用机制.方法 MTT法研究CSA对人肝癌HepG2的杀伤作用;荧光显微镜观察HepG2细胞形态;流式细胞仪研究CSA对HepG2细胞的凋亡诱导作用;激光共聚焦显微镜检测CSA对HepG2细胞内Ca~(2+)]i的影响.结果 CSA对人肝癌HepG2有明显细胞毒性作用,并且有剂量依赖性,IC_(50)为142.82 μg/mL;HepG2细胞在CSA作用后出现特征性凋亡形态特征,凋亡细胞比率明显高于自然凋亡率;且阻断细胞由S期向G_2期的移行,CSA明显升高HepG2细胞Ca~(2+)]i,并与药物质量浓度呈量效正相关.结论 CSA对人肝癌HepG2有明显的杀伤和促凋亡作用,其机制可能与CSA造成肿瘤细胞内钙离子超载有关.
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| 关 键 词： | 野西瓜总生物碱 人肝癌HepG2细胞 细胞凋亡 钙超载 |
| 收稿时间： | 2008/8/10 0:00:00 |
Relationship between apoptosis and [Ca2+]_i in HepG2 induced by Capparis spinosa alkaloid |
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| YU Lei,MO Ke,WANG Wei,CUI Rong-tian,ZOU Xiang and JI Yu-bin.Relationship between apoptosis and [Ca2+]_i in HepG2 induced by Capparis spinosa alkaloid[J].Chinese Traditional and Herbal Drugs,2009,40(2):255-258. |
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| Authors: | YU Lei MO Ke WANG Wei CUI Rong-tian ZOU Xiang and JI Yu-bin |
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| Institution: | School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China;School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China;School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China;Research Center of Life and Environment Science, Harbin University of Commerce, Harbin 150076, China;Engineering Research Center of Natural Anti-cancer Drugs, Ministry of Education, Harbin 150076, China |
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| Abstract: | Objective To study the mechanism of killing and apoptosis-inducing effects of Capparis spinosa alkaloid (CSA) on human hepatocarcinoma cell line HepG2. Methods The killing effect of CSA on human hepatocarcinoma cell line HepG2 was studied by MTT method. Morphological observation of HepG2 cells was carried out by fluorescence microscope. Results The CSA had obvious cytotoxicity on the HepG2 in a dose-dependent manner and its IC_(50) value was 142. 82 μg/mL. The HepG2 cells showed the characteristic morphologic changes of apoptosis by the function of CSA and the apoptosis percentage is higher than that of the natural one. The progress of cells cycle from S phase to G_2 phase had been blocked by CSA. The intracellular Ca~(2+) level had been increased by the function of CSA, which was positively related with drug concentration. Conclusion CSA has obviously killing and apoptosis-inducing effects on human hepatocarcinoma cell line HepG2 and calcium overload might also be invovled in these events. |
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| Keywords: | Capparis spinosa L alkaloid (CSA) human hepatocarcinoma cell line HepG2 apoptosis calcium overload |
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