PDGFRA在肺癌细胞系中的表达及其对细胞增殖和侵袭能力的影响

目的:明确血小板源性生长因子受体A(platelet-derived growth factor receptor alpha,PDGFRA)对肺癌细胞增殖和侵袭能力的影响.方法:在8种肺癌细胞系以及正常肺细胞HBE中通过RT-PCR方法检测PDGFRA的基本表达水平,分别选取PDGFRA表达高的两组肺癌细胞系(A549)通过慢病毒转染构建稳定knock down和过表达PDGFRA细胞系,再进一步通过MTS,划痕实验,transwell实验以及western-blot实验明确PDGFRA对肺癌细胞增殖和侵袭能力的影响.结果:与对照组相比,过表达PDGFRA后,细胞增殖以及侵袭能力显著增加,同时与侵袭能力相关的细胞黏附分子E-cadherin和细胞骨架蛋白Vimentin表达上调,划痕实验和transwell实验结果表明PDGFRA能够促进细胞侵袭能力.在knock down PDGFRA细胞系中,细胞增值能力降低,细胞黏附分子E-cadherin和细胞骨架蛋白Vimentin表达下调,与对照组相比,细胞侵袭能力下降.结论:PDGFRA能够促进肺癌细胞增殖并有助于肺癌细胞的侵袭转移.

Effect of PDGFRA on Cell Proliferation and Invasion in Lung Cancer Cell Lines

Objective:To evaluate the effect of platelet derived growth factor receptor alpha( PDGFRA) on the proliferation and invasion of lung cancer cells. Methods: The mRNA expression level of PDGFRA in eight lung cancer cell lines and normal lung cell HBE was detected by PT-PCR. Two groups of lung cancer lines( A549) from the high expression level of PDGFRA were selected, then stable knock down and over-ex-pression PDGFRA cell lines were constructed through lentiviral transfection. Furthermore, the effect of PDG-FRA on cell proliferation and invasion in these cells were evaluated by MTS, scratch test, Transwell and west-ern-blot test. Results: Over-expression of PDGFRA showed that cell proliferation and invasion significantly increased, meanwhile, E-candherin and Vimentin also increased, scratch test and transwell revealed that PDGFRA significantly improved the cell invasion. Whereas, knock down of PDGFRA decreased the cell prolif-eration, the expression of E-candherin and Vimentin decreased and the cell invasion also significantly also de-creased compared with the control group. Conclusion: PDGFRA can promote the cell proliferation and con-tribute to the cell invasion in lung cancer cells.