苦瓜乙醇提取物对肥胖大鼠糖代谢和内脏脂肪的影响

目的 研究苦瓜乙醇提取物对喂饲高脂饲料所致肥胖大鼠糖代谢和内脏脂肪量的影响.方法 高脂饲料饲养制备肥胖大鼠模型.肥胖大鼠随机分为模型组,苦瓜乙醇提取物低、中、高剂量(9、18、36 g/kg)组,左旋肉碱(600 mg/kg)阳性对照组,另设对照组(正常饲料饲养)和苦瓜乙醇提取物36 g/kg给药组.各组大鼠每天ig相应药物或等体积0.5％羧甲基纤维素钠溶液,每天记录摄食量,每周记录体质量.给药第6周进行糖耐量实验:给药7周后所有动物禁食18h,麻醉后腹主动脉采血,检测血清葡萄糖和胰岛素水平;分离附睾、肾周和肠系膜脂肪并称质量,附睾脂肪组织随后进行HE染色检测脂肪细胞病变.结果 苦瓜乙醇提取物36 g/kg能够明显降低肥胖大鼠体质量,抑制大鼠食物利用率,减少附睾、肾周和肠系膜白色脂肪量,降低空腹血糖浓度,抑制附睾脂肪细胞肥大;苦瓜乙醇提取物18 g/kg也明显降低肥胖大鼠附睾脂肪质量.但苦瓜乙醇提取物对肥胖大鼠的糖耐量和胰岛素抵抗指数无明显影响.结论 苦瓜乙醇提取物通过抑制肥胖大鼠内脏脂肪聚积和附睾脂肪细胞肥大以及降低空腹血糖浓度发挥减肥和抗糖尿病作用.

Effects of ethanol extract from Momordica charantia on glycoMetabolism and visceral fat in obese rats

Objective To study the effects of ethanol extract from Momordica charantia(EEMC) on glycometabolism and visceral fat in obese rats fed with high-fat diet(HFD).Methods After 5-week HFD induction,the obese rat model was established successfully.The obese rats were divided into model group,EEMC low-,midium-,and high-dose(9,18,and 36 g/kg) groups,and L-carnitine 600 mg/kg group.The rats fed with normal diet were randomly divided into control group and EEMC(36 g/kg) group.The rats in different groups were treated with 0.5% CMC-Na or ig administrated with the same volume of EEMC respectively.The daily food intake and weekly body weight of rats were recorded.Oral glucose tolerance experiment was performed at the sixth week after the administration;Then one week later,all the rats were fasted for 18 h,and the serum samples from abdominal arteria of rats were harvested for the levels of glucose and insulin determination.The epididymal,perirenal,and mesentery white adipose tissue were collected and weighed.At last the adipocyte hypertrophy of epididymal white adipose tissue was determined with HE staining.Results The body weight,food utilization,fasting blood glucose,and weight of epididymal,perirenal,and mesentery white adipose tissue were obviously decreased by high-dose of EEMC and the adipocyte hypertrophy induced by high fat diet was also mitigated.Epididymal white adipose tissue weight was also reduced by midium dose of EEMC.But the glucose tolerance and insulin resistance index were not influenced by EEMC.Conclusion The anti-obesity and antidiabetic effects of EEMC in obese rats were mediated by inhibiting visceral fat accumulation and adipocyte hypertrophy of epididymal white adipose tissue and decreasing fasting blood glucose.