实时荧光核酸恒温放大检测（SAT）法对肺结核诊断价值的研究

目的 检测实时荧光核酸恒温放大检测（SAT）法对结核病诊断的作用。 方法 收集172例疑似肺结核患者的痰液标本2份/例，1份进行涂涂片金-胺染色，另一份进行罗氏培养基培养与菌种鉴定及SAT法检测结核分枝杆菌，如SAT法和培养结果不符合则使用痰荧光定量PCR法（FQ-PCR)进行复核。 结果 172例入组患者中有156例根据临床及实验室结果确诊为肺结核病。如果以Mtb培养鉴定结果和荧光PCR结果作为判定金标准，SAT检测的敏感度和特异度分别为97.8%（89/91）和97.5%（79/81）。如以临床诊断为标准，SAT诊断结核的敏感度为58.3%（91/156），特异度为100.0％（16/16）。对于痰菌阳性肺结核患者，SAT 阳性率为84.9%（79/93）；对于痰菌阴性患者，SAT的阳性率为19.0%（12/63）。 结论 SAT法可以作为临床对结核病的辅助诊断方法。

Diagnostic value of simultaneous amplification and testing in patients with pulmonary tuberculosis

Objective To evaluate the value of simultaneous amplification and testing(SAT) in diagnosis of patients with pulmonary tuberculosis.Methods Sputum smear,culture with species identification,and TB-SAT were studied in 172 patients with suspected pulmonary tuberculosis.The samples,which had inconsistent results between culture and SAT,should be rechecked with fluorescent quantitative PCR(FQ-PCR).Results 156 patients were clinically diagnosed with pulmonary tuberculosis.If taking the culture plus FQ-PCR as the gold stan-dard,the sensitivity and specificity of TB-SAT were 97.8%(89/91) and 97.5%(79/81),respectively.However,the sensitivity and specificity of TB-SAT were 58.3%(91/156)and 100.0%(16/16)with the clinical diagnosis as stan-dard.The positive rates of TB-SAT were 84.9%(79/93) for sputum positive patients and 19.0%(12/63) for sputum negative patients.Conclusion TB-SAT is an effective method for diagnosis of patients with pulmonary tuberculosis.