刺五加化学成分及自由基清除活性研究

目的研究长白山区植物刺五加的化学成分及其自由基清除活性。方法采用硅胶柱色谱、ODS柱色谱、半制备HPLC等色谱方法进行分离纯化,通过波谱技术鉴定其结构,并对化合物进行自由基清除活性测定。结果从刺五加中分离得到了11个化合物,分别鉴定为邻苯二甲酸二(2-乙基)己酯(1)、邻苯二甲酸二异丁酯(2)、落叶松脂醇(3)、tripterygiol(4)、8,8′-bisdihydrosiringenin(5)、异嗪皮啶(6)、丁香脂素(7)、蛇菰宁(8)、3,3′-dimethoxy-4,8′-oxyneoligna-9,4′,7′,9′-tetraol(9)、1-(4′-hydroxy-3′-methoxyphenyl)-2-4″-(3-hydroxypropyl)-2″,6″-dimethoxyphenoxy]propane-1,3-diol(10),芝麻脂素(11)。结论化合物1～5和8～11为首次从刺五加中得到,且发现化合物3、4、6和7具有较好的自由基清除活性,其对DPPH清除作用的IC50值分别为0.01、0.01、0.01和0.66μg/mL,可为发现抗氧化和抗衰老药物先导化合物提供参考。

Chemical constituents of Acanthopanax senticosus and their free radical scavenging activities

Objective To study the chemical constituents of Acanthopanax senticosus from Changbai Mountain area and their free radical scavenging activities.Methods The chromatography on silica gel column,ODS column,and semi-preparative HPLC were used to isolate and purify the constituents and their structures were identified on the basis of spectral data analyses.Also the free radical scavenging activities of the compounds were evaluated.Results Eleven compounds were obtained from A.senticosus and were identified as bis(2-ethylhexyl) phthalate(1),diisobutyl phthalate(2),lariciresinol(3),tripterygiol(4),8,8′-bisdihydrosiringenin(5),isofraxidin(6),syringaresinol(7),balanophonin(8),3,3′-dimethoxy-4,8′-oxyneoligna-9,4′,7′,9′-tetraol(9),1-(4′-hydroxy-3′methoxyphenyl)-2-4″-(3-hydroxypropyl)-2″,6″-dimethoxyphenoxy] propane-1,3-diol(10),and sesamin(11).Conclusion Compounds 1—5 and 8—11 are obtained from A.senticosus for the first time.Compounds 3,4,6,and 7 show the remarkable scavenging activities toward DPPH free radical with IC50 values of 0.01,0.01,0.01,and 0.66 μg/mL,respectively,which could provide the reference for the development of leading compounds in anti-oxidant and anti-aging aspects of the drugs.