藿香正气水抑菌作用药效物质基础及质量控制方法研究

目的探讨藿香正气水ig大鼠后肠道内容物指纹图谱特征峰所代表的化学成分与抑菌药效的相关性,为建立"代谢物指纹图谱与药效关联"的中药质量控制模式提供理论依据和数据支持。方法自制11批藿香正气水,ig给予大鼠后40min处死动物,制备肠道内容物样品,检测藿香正气水抑菌效果,建立HPLC指纹图谱,并用化学计量学方法将抑菌圈直径与指纹图谱数据进行相关分析,以期寻找控制藿香正气水质量的药效成分群。结果以强迫引入法进行多元线性回归分析,有10个与抑菌活性密切相关的色谱峰被引入回归方程,其中呈正相关的色谱峰有4个(6、13、26、30号),且26号峰(和厚朴酚)经实验证明具有抑菌作用。结论运用所建立的数学模型找到可控制藿香正气水质量的体内药效成分群,且证明相关成分具有抑菌作用,这些成分群对藿香正气水质量控制具有参考价值,该法切实可行。

Antibacterial material basis and quality control of Huoxiang Zhengqi Tincture

Objective After ig administration of Huoxiang Zhengqi Tincture(HZT) to rats,the correlation between the homologous chemical composition from intestinal contents represented by fingerprint characteristic peaks and antibacterial efficacy was investigated to provide the theoretical basis and data for the model of quality control in Chinese materia medica by establishing "the fingerprint of metabolites association with efficacy".Methods Eleven batches of HZT were self-designed and rats were killed in 40 min after ig administration.The intestinal contents samples were obtained,the antibacterial effect was determined,the HPLC fingerprint was established,and simultaneously the correlation analysis between the inhibition zone diameter and fingerprint data was conducted by chemometric methods,so as to find the effictive component group that could control the quality of HZT.Results Multiple linear regression analysis was carried out.Ten peaks related to the antibacterial activity were closely inducted to the regression equation,among which peaks 6,13,26,and 30 were positively correlated,and peak 26 showed the antibacterial effect proved by the experiments.Conclusion The component groups with curative effect in vivo which could control the quality of HZT are basically achieved by applying mathematical model.In addition,the relevant components prove to have antibacterial effect and the method is feasible.