小柴胡汤化学成分及其在抑郁模型大鼠体内代谢成分的分析

目的采用UPLC-MS/MS技术分析小柴胡汤提取液化学成分及其在抑郁模型大鼠体内的代谢产物,为其抗抑郁作用药效物质研究奠定基础。方法采用Acquity UPLCTMBEH C18柱进行色谱分离,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,以电喷雾质谱检测,扫描范围为m/z 150～1 500,在正负离子同时扫描的模式下,通过获得一、二级质谱信息,参照对照品及相关文献信息,确定小柴胡汤的化学成分及其在抑郁模型大鼠体内的代谢产物的可能结构。结果小柴胡汤样品中共检测到44个化学成分。抑郁模型大鼠ig给予小柴胡汤后,在血清样品中共检测出7个原形成分和8个代谢产物;在尿液样品中共检测出12个原形成分和19个代谢产物。结论建立的UPLC-MS/MS法能较全面地分析小柴胡汤的化学成分及其在抑郁模型大鼠体内的代谢产物,为进一步研究小柴胡汤抗抑郁作用药效物质基础提供依据。

Analysis on chemical constituents in Xiaochaihu metabolites in depressed rats Decoction and their in vivo

Objective To study the chemical constituents in Xiaochaihu Decoction(XCD) and their in vivo metabolites in depressed rats by UPLC-MS/MS and lay the fundation for the investigation of antidepressant effects.Methods The analysis was performed on an Acquity UPLCTM BEH C18 column with gradient elution system of acetonitrile and water containing 0.1% formic acid.Using electrospray ionization(ESI) in both positive and negative ion modes with scanning range from m/z 150 to 1 500.The components in extract of XCD and their in vivo metabolites in depressed rats were identified by comparing the MS and MS2data with the reference substances and related literature.Results Forty-four components were identified in XCD extract.In bioanalysis process in vivo,seven original components and eight metabolites were detected in the serum,and 12 original components and 19 metabolites in the urine after ig administration.Conclusion The established UPLC-MS/MS method could comprehensively analyze the components in XCD and metabolites in depressed rats so as to provide the basis for study on the pharmacodynamic material basis of their antidepressant effects.