中药活性小分子人工抗原合成的技术要点

中药免疫分析技术适用于中药复杂组分的研究,基于此技术的研究思路与方法越来越引起研究者的重视.免疫分析的前提是抗体制备,中药活性小分子人工抗原的合成是中药活性成分抗体制备的关键步骤.从载体的选择、交联方法的选择、纯化及鉴定方法、合成条件的优化及影响免疫原性的因素等方面,对中药活性小分子人工抗原合成的技术要点进行综述,从而为中药免疫分析技术的建立和发展奠定基础.

Key techniques in artificial antigen synthesis of active small molecule in Chinese materia medica

Immunoassay technology is suitable for the study on complex components in Chinese materia medica(CMM).Research ideas and methods based on this technology have drawn the increasing attention of researchers.The premise of the immunoassay is antibody preparation and the critical step of antibody preparation of active ingredients in CMM is the artificial antigen synthesis of active small molecule in CMM,of which the key technology is summarized in this article,including carrier choice,methods for crosslinking,purification and identification of artificial antigens,the optimization of the synthesis conditions as well as factors influencing the immunogenicity.This article is supposed to lay the foundation for the establishment and development of CMM immunoassay technology.