馥芳艾纳香快繁体系的建立

目的建立馥芳艾纳香Blumea aromatica的快繁技术体系,为大量生产种苗提供基础。方法利用不同激素配比的培养基,筛选出馥芳艾纳香快速繁殖的最适培养基。结果带腋芽茎段是丛生芽诱导的最佳材料;丛生芽诱导的最适培养基为MS+2.0 mg/L 6-BA+0.2 mg/L NAA,诱导率为100%;最佳继代增殖培养基为MS+2.0 mg/L 6-BA+0.5 mg/L KT+0.2mg/L NAA,增殖倍数为6.83;最适的生根培养基为1/2 MS+0.3 mg/L NAA,生根率100%,移栽成活率84.07%。最适培养条件为温度26℃、光照强度为2 000 lx、光照时间10 h。结论快繁技术体系可在短时间内提供大量种苗,并为馥芳艾纳香规模化生产种苗提供技术指导。

Establishment of rapid propagation system of Blumea aromatica

Objective To establish a rapid propagation system of Blumea aromatica through tissue culture technique for large-scale seedlings.Methods Using media with different hormones proportion to optimize the tissue culture of B.aromatica.Results Stems with axillary bud was the suitable explant for inducing and the cluster buds inducing medium consisted of MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA with inductivity of 100%.The best medium for subculture proliferation was MS +2.0 mg/L 6-BA +0.5 mg/L KT +0.2 mg/L NAA with proliferation times of 6.83.The optimal medium for rooting was 1/2 MS +0.3 mg/L NAA with rooting and survival rates of 100% and 84.07%,respectively.The optimal cultural condition was temperature 26 ℃,light intensity 2 000 lx,and illumination time 10 h.Conclusion The tissue culture technique and rapid propagation system of B.aromatica could be used for large-scale seedlings in short time and provide technical guidance for large-scale production.