采用ISSR和SRAP技术评价浙南忍冬属药材遗传多样性

目的 评价浙南忍冬属LoniceraL.药材遗传多样性.方法 采用ISSR和SRAP分子标记技术检测5种忍冬属药材遗传多样性,NTSYS软件处理数据,UPGMA构建聚类图;Mantel检测法对2种标记进行相关性检验;用引物分辨力(RP)、多态性条带比率(PPB)等参数对标记效率进行评价.结果 16条ISSR引物和22对SRAP引物分别扩增出232、215条带;UPGMA可将5种忍冬属药材聚为2大类,一类为金银花基原植物,另一类则为山银花基原植物,两种标记技术相关系数(r)为0.970 3.结论 ISSR和SRAP均可有效地分析忍冬属药材资源的遗传多样性,且ISSR标记技术优于SRAP.

Evaluation on genetic diversity among species of Lonicera L. in southern Zhejiang Province by ISSR and SRAP

Objective To study the genetic diversity among species of Lonicera L.in southern Zhejiang Province.Methods Genetic diversity among five species in Lonicera L.was estimated by ISSR and SRAP techniques.Data were analyzed by NTSYS and the system diagram of genetic relationship was constructed by UPGMA.Mantel test was used to determine the correlation of ISSR and SRAP analyses.Labeling efficiency was evaluated by using different parameters such as resolution of primer(RP) and polymorphic bands(PPB).Results A total of 232 and 215 bands were amplified respectively by using 16 ISSR primers and 22 primer combinations of SRAP.Five species of Lonicera L.were clustered into two groups,one is the original plant of Lonicerae Japonicae Flos,and the other is the original plant of Lonicerae Flos.The correlation coefficient(r) between ISSR and SRAP was 0.970 3.Conclusion ISSR and SRAP could be used to effectively analyze the genetic diversity among species of Lonicera L.and ISSR is more efficient than SRAP.