艾迪注射液的化学成分研究

目的 研究艾迪注射液的化学成分.方法 利用反相半制备液相色谱、Sephadex LH-20凝胶柱色谱等方法分离纯化,并通过光谱数据鉴定化合物结构.结果 分离得到22个化合物,分别鉴定为3-O-(3′,4′-二乙酰氧基)-β-D-吡喃木糖基-6-O-β-D-吡喃葡萄糖基-环黄芪醇(1)、黄芪甲苷(2)、黄芪皂苷Ⅱ (3)、黄芪皂苷Ⅰ(4)、异黄芪皂苷Ⅰ(5)、乙酰黄芪皂苷Ⅰ(6)、人参皂苷Re (7)、人参皂苷Rf(8)、人参皂苷Rg1 (9)、人参皂苷Rb3 (10)、三七皂苷R4 (11)、人参皂苷Rb1 (12)、人参皂苷Rc (13)、人参皂苷Rb2 (14)、人参皂苷Rd (15)、丝瓜苷H(16)、3-O-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃葡萄糖基(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡萄糖(1→6)-β-D-吡喃葡萄糖酯苷(17)、3-O-β-D-吡喃葡萄糖基(1→3)-α-L-吡喃鼠李糖[β-D-吡喃葡萄糖基-(1→4)]-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡萄糖(1→6)-β-D-吡喃葡萄糖酯苷(18)、紫丁香苷(19)、刺五加皂苷E(20)、4-(1,2,3-三羟基丙基)-2,6-二甲氧基苯-1-O-β-D-葡萄糖苷(21)、松柏苷(22).结论 经LC-MS分析检测,化合物1～6为黄芪中的化学成分,化合物7～18为人参中的化学成分,化合物19～22为刺五加中的化学成分,其中化合物1为新化合物,命名为新黄芪皂苷Ⅰ.

Studies on chemical constituents from Aidi Injection

Objective To investigate the chemical constituents from Aidi Injection.Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparation HPLC repeatedly,and their structures were identified by spectral data.Results Twenty-two compounds were isolated and identified as 3-O-(3′,4′-diacetyl)-β-Dxylopyranosyl-6-O-β-D-glucopyranosyl-cycloastragenol(1),astragaloside IV(2),astragaloside II(3),astragaloside I(4),isoastragaloside I(5),acetylastragaloside I(6),ginsenosid-Re(7),ginsenoside-Rf(8),ginsenoside-Rg1(9),ginsenoside-Rb3(10),notoginsenoside-R4(11),ginsenoside-Rb1(12),ginsenoside-Rc(13),ginsenoside-Rb2(14),ginsenoside-Rd(15),lucyoside H(16),3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl oleanolic acid 28-Oα-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(17),3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl [β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(18),syringin(19),elentheroside E(20),4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl1-O-β-D-glucopyranoside(21),and coniferin(22).Conclusion LC-MS analysis shows that compounds 1—6 are originated from Astragalus membranceus,compounds 7—18 from Panax ginseng,and compounds 19—22 from Acanthopanacis senticosi.Compound 1 is a new compound named neoastragaloside I.