黑龙江产万寿菊花醇提物的指纹图谱研究

目的建立黑龙江产万寿菊花醇提物的指纹图谱并定性分析共有峰,为万寿菊花的质量控制提供参考。方法采用HPLC分离UV检测建立色谱指纹图谱,Zorbax Eclipse XDB C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.2%磷酸水溶液梯度洗脱,体积流量1 mL/min,柱温25℃,检测波长254 nm;用LC-MS定性共有峰,电喷雾离子源(ESI),负离子模式扫描,雾化器压力241.325 kPa,干燥器温度350℃,干燥气体积流量10 L/min,扫描范围:m/z 50～1 000。结果万寿菊花醇提物的指纹图谱有8个共有峰,并具有较好的精密度、重复性和稳定性,其中5个共有峰所对应的化学物质为万寿菊素、异槲皮素、异槲皮素苷和槲皮万寿菊素及其苷。结论建立的万寿菊花醇提物的指纹图谱,为万寿菊花的质量控制提供参考。

Fingerprint for methanol extract of Tagetes erecta flowers from Heilongjiang

Objective To establish fingerprint and qualitatively analyze the common peaks for the methanol extract of Tagetes erecta flowers from Heilongjiang Province,which could provide reference for the quality control of T.erecta.Methods The fingerprint was established with UV detection and HPLC separation.Chromatographic condition included a Zorbax Eclipse XDB column(250 mm × 4.6 mm,5 μm),gradient elution with acetonitrile-0.2% phosphoric acid as mobile phase,flow rate 1 mL/min,column temperature 25 ℃,and detection wavelength 254 nm.LC-MS was used for the qualitative analysis of common peaks.LC-MS performed on electrospray ionization(ESI) source with anion-pattern scanning and atomizer-pressure 241.325 kPa as well as desiccator temperature 350 ℃,flow rate 10 L/min,and scanning area m/z 50-1 000.Results The fingerprint of the alcohol extract from T.erecta flowers with good precision,repeatability,and stability composed of eight common peaks.The chemical compositions corresponding to five common peaks were patuletin,isoquercitin,isoquercitrin,and quercetagetin with its glycosides.Conclusion The liquid fingerprint established could offer reference for the quality control of T.erecta.