44例儿童急性淋巴细胞白血病免疫表型分析

目的探讨白细胞分化抗原在急性淋巴细胞白血病(ALL)免疫分型中的意义。方法应用三色流式细胞术对44例FAB分型为ALL的患儿骨髓进行免疫分型。结果44例ALL中B细胞系急性淋巴细胞白血病(B-ALL)32例,占72.7%,T细胞系急性淋巴细胞白血病(T-ALL)8例,占18.2%,T/B双表型4例,占9.1%,其中在B-ALL中CD19(100%)敏感性最高,cCD79a特异性强,明显高于cCD22(31.25%,P<0.05);在T-ALL中CD7(100%)和cCD3(100%)敏感性最高,cCD3特异性强;HLA-DR、CD34、CD38等非系列相关抗原在儿童ALL中均有较高表达。ALL伴髓系抗原表达22例,主要表达CD33、CD13。结论儿童ALL以B-ALL为主,CD19和cCD79a是B-ALL最具有诊断价值的抗原。CD7和cCD3是T-ALL最具有诊断价值的抗原。儿童ALL具有较高的CD34、CD38和髓系抗原表达。

Analysis of immunophenotype of children acute lymphoblastlc leukemia

Objective To investigate the clinical significance of immunophenotype of children acute lymphoblastic leukemia.Methods Immunophenotype of bone marrow samples diagnosed to ALL by FAB was analyzed with 3-color flow cytometry in 44 patients.Results In 44 cases of ALL,there were 32 cases of B-ALL with the proportion of 72.7%,8 cases of T-ALL with the proportion of 18.2 %,and 4 cases of T/B-ALL with the proportion of 9.1% via immunophenotyping.In B-ALL CD19 was the most susceptible,cCD79a was with intense specificity and obviously surpassed cCD22(31.25%,P<0.05).In T-ALL cCD3 and CD7 were the most sensitive,and cCD3 was intense in specificity.un-Serial antigens including HLA-DR,CD34 and CD38 had high expression.My -ALL made up 50% of ALL,whose primary expressions were such myeloid antigens as CD13 and CD33.Conclusion B-ALL is main in ALL,CD19 and cCD79 are the most valuable antigens in B-ALL,whereas CD7 and cCD3 is most essential in T-ALL.Besides,CD34,CD38 and myeloid antigens are highly expressimved in children ALL.