| 南京地区产超广谱β-内酰胺酶菌的流行病学调查 |
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| 引用本文： | 王卫文,李有武,夏永祥,刘才冬. 南京地区产超广谱β-内酰胺酶菌的流行病学调查[J]. 医学理论与实践, 2012, 0(22): 2751-2752 |
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| 作者姓名： | 王卫文 李有武 夏永祥 刘才冬 |
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| 作者单位： | 南京医科大学附属南京第一医院检验科 |
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| 摘 要： | 目的:了解南京地区产ESBLs菌基因型的流行情况。方法:用TEM型、SHV型、CTX-M型、OXA型ESBLs的特定引物进行扩增并对扩增产物进行测序分析确定基因型。结果:30株产ESBLs大肠埃希菌和30株产ESBLs肺炎克雷伯菌未发现TEM型、SHV型、OXA型ESBLs,51株产CTX-M型ESBLs,主要为CTX-M-3、CTX-M-14。9株未扩增到ESBLs基因型。结论:南京地区产ESBLs菌基因型主要以CTX-M型为主。
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| 关 键 词： | 超广谱β-内酰胺酶 基因型 |
The Prevalence of the Genotypes of Producing Extended-spectrum Beta-lactamases Strains in Nanjing Area |
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| Affiliation: | WANG Weiwen,LI Youwu,XIA Yongxiang,et al.Affiliated Nanjing First Hospital,Nanjing Medical University,Nanjing City,Jiangsu Province 210006 |
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| Abstract: | Objective:To investigate the prevalence of the genotypes of producing extended-spectrum beta-lactamases(ESBLs) stains in Nanjing area.Methods:PCR was used to determine the presence of TEM,SHV,CTX-M and OXA of ESBLs by the specific primer.Then we detected the amplification products to determine their genotypes.Results:Among 30 strains of Escherichia coli and 30 strains of Klebsiella pneumoniae we didn't find TEM,SHV,CTX-M and OXA type ESBLs.51 stains were detected CTX-M type ESBLs,they are mainly CTX-M-3,CTX-M-14.9 stains were negative in PCR.Conclusion:The main genotype was CTX-M type ESBLs in the ESBLs-producing strains in Nanjing area. |
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| Keywords: | Extended-spectrum beta-lactamases Genotype |
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