酚红外排水平评价肺上皮细胞多药耐药相关蛋白1的功能

目的：以酚红为多药耐药相关蛋白1（MRP1）的底物,丙磺舒为其抑制剂,通过酚红的外排水平建立评价肺上皮细胞MRP1的功能的方法。方法：小鼠静脉注射酚红（250mg/kg）,30min后进行肺泡灌洗,测定灌洗液中酚红的浓度,并以雾化吸入MRP1特异性抑制剂丙磺舒（150mg/kg）作为对照,观察给予抑制剂前后灌洗液中酚红浓度及灌洗液中酚红浓度/血浆中酚红浓度比值的变化,来评价小鼠肺上皮细胞MRP1功能的改变。结果：与未给予抑制剂相比,给予MRP1抑制剂丙磺舒后,血浆中酚红浓度无显著性差异,但与未给予抑制剂相比,给予MRP1抑制剂丙磺舒60min后,其肺泡灌洗液中酚红浓度、灌洗液中酚红浓度/血浆中酚红浓度比值显著性降低。结论：肺泡灌洗液中酚红的外排水平反映小鼠肺上皮细胞MRP1功能的改变。

Evaluation on the function of MRP1 in pulmonary epithelial cells by the efflux level of phenolsulfonphthalein

AIM： To evaluate the function of MRP1 in pulmonary epithelial cells by the efflux level of suhstrate using pheno[sulfonphthalein as MRP1 substrate and prohenecid as MRPl-specif- ic inhibitor. METHODS： The mouse was divided into the control group and inhibitor inhaled groups. The bronchoalveolar lavage fluid （BALF） and plasma samples were collected at 30 rain after phenolsulfonphthalein （250 mg/kg） was administered to the intravenous injection. control group mouse by The inhibitor inhaled groups mouse was administered phenolsulfonph- thalein （250 mg/kg） at 10, 30 and 60 min after inhaled probenecid （150 mg/kg）, and the BALF and plasma samples were collected at 30 min af- ter phenolsulfonphthalein administration. The phenolsulfonphthalein concentration in BALF and plasma was determined and the concentra- tion of in BALF/plasma ratio was calculated. RESULTS：There was no difference in the plasma concentration of phenolsulfonphthalein between the control group and inhibitor groups, Com- pared with control group, the phenolsulfonph- thalein concentration in BALF and the phenol- sulfonphthalein concentration of in BALF/plas- ma ratio of the 60 min after inhaled probenecid group had significant difference. CONCLUSION： The efflux level of phenolsulfonphthalein in BALF could reflect the function of mouse pul- monary epithelial cells MRP1.