慢性丙型肝炎患者血清IL-21水平检测及临床意义

目的：检测慢性丙型肝炎（CHC）患者血清白细胞介素21（IL-21）水平及肝功能、HCV-RNA,探讨血清IL-21水平变化与肝功能各项参数及HCV-RNA的关系,研究IL-21在慢性丙型肝炎发病中的作用。方法：双抗体夹心（ELISA）法检测27例慢性丙型肝炎患者和10例正常对照者血清中IL-21的水平。同时检测患者HCV-RNA、肝功能等指标,并与患者的其他临床数据进行统计分析。结果：慢性丙型肝炎患者血清IL-21水平显著高于正常对照组（P〈0.01）;ALT升高的慢性丙型肝炎患者血清IL-21显著高于ALT正常的慢性丙型肝炎患者,且其血清IL-21水平与ALT、AST显著正相关（分别为r=0.647,P〈0.01;r=0.591,P〈0.01）;慢性丙型肝炎患者血清IL-21水平与HCV-RNA水平无显著相关性。结论：IL-21与慢性丙型肝炎肝脏炎症密切相关,是反应慢性丙型肝炎炎症程度的灵敏指标。

Serum IL-21 levels in patients with chronic hepatitis C and clinical significance of IL-21

AIM. To determine serum inter- leukin-21 （IL-21） level and evaluate the roles of IL-21 in chronic hepatitis C （CHC）. METH-ODS： Serum IL-21 levels of 27 CHC patients and 10 control subjects were determined by enz- ymelinked immunosorbent assay （ELISA） and the relationship between serum IL-21 levels and HCV-RNA, the live biochemical parameters were studied. RESULTS： Serum IL-21 levels in CHC patients were significantly higher than those in control subjects （P〈0.01）. Especially, CHC patients with elevated alanine aminotrans- ferase （ALT） have higher IL-21 than patients with normal ALT. There were highly significant positive associations between serum IL-21 and ALT （r=0. 647, P〈0.01） as well as aspartateaminotransferase （AST） （r=0. 591, P〈0.01）. But no association was observed between IL-21 and HCV-RNA. CONCLUSION. IL-21 is a cyto- kine involved in HCV-associated liver pathologi- cal damage and may be an available marker to e- valuate hepatic inflammation for CHC.