MLC及MLCK在子宫肌瘤组织中的表达及意义

目的:探讨MLC及MLCK在子宫肌瘤发病机制中的作用。方法:采用免疫组化、RT-PCR及Western blot方法检测30例子宫肌瘤及其附近正常平滑肌组织中MLC、MLCK及p-MLCK的表达。结果:免疫组化检测MLC、MLCK及p-MLCK在子宫肌瘤组织中阳性表达水平均高于平滑肌组织,差异有统计学意义(P<0.01)。RT-PCR显示MLC及MLCK mRNA在子宫肌瘤组织中表达上调,差异有统计学意义(P<0.01)。经Western blot检测MLC、MLCK及p-MLCK蛋白含量在子宫肌瘤组织中显著高于平滑肌组织,差异有统计学意义(P<0.05)。结论:MLC及MLCK在平滑肌及肌瘤组织中表达异常,提示二者可能参与了子宫肌瘤的发生与发展,推测其可能与细胞收缩异常相关。

Expression and significance of MLC and MLCK in uterine myoma tissue

Objective:To investigate the expressions of MLC and MLCK in uterine myoma and discuss the role of them in pathogenesis of uterine myoma.Methods:Immunohistochemistry,RT-PCR,Western blot were used to determine the expressions of MLC,MLCK and p-MLCK in uterine myoma and adjacant normal smooth muscle tissue.Results:① Immunohistochemistry results revealed that MLC,MLCK and p-MLCK in uterine myoma were significantly higher correlated with smooth muscle tissue(P<0.001).② RT-PCR results displayed MLC and MLCK mRNA in uterine myoma were up-regulated compared with smooth muscle tissue,the differences were significant(P<0.001).③ According to western blotting data,MLC,MLCK and p-MLCK protein content in uterine myoma were higher than those of smooth muscle tissue(P<0.05).Conclusion:MLC and MLCK in uterine smooth muscle and myoma are expressed abnormally,which indicates that both of them may be involved in the occurrence and development of uterine myoma,and they may correlate with the cell anomal contraction.