| 4EBP1和P70S6K在不同宫颈病变组织中的表达及其意义 |
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| 引用本文： | 朱旭明,王鲁文,董延华,张秀娟,马双玲. 4EBP1和P70S6K在不同宫颈病变组织中的表达及其意义[J]. 中国妇幼保健, 2013, 28(12): 1959-1962 |
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| 作者姓名： | 朱旭明 王鲁文 董延华 张秀娟 马双玲 |
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| 作者单位： | 郑州大学第三附属医院妇产科,河南郑州,450052 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目经费支持(项目编号：122300410043) |
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| 摘 要： | 目的:研究真核细胞翻译起始因子4E结合蛋白(4EBP1)及核糖体40S小亚基S6蛋白激酶(P70S6K)在不同宫颈病变组织中的表达水平及其在宫颈癌发生、发展中的作用。方法:采用免疫组化SP法及PT-PCR法检测宫颈癌、宫颈上皮内瘤变(CIN)及正常宫颈组织中的4EBP1和P70S6K表达水平。结果:在正常宫颈、CIN及宫颈癌组织中,4EBP1和P70S6K蛋白及mRNA水平表达依次增高,且3组比较差异有统计学意义(P<0.05)。4EBP1和P70S6K在宫颈癌中的表达水平呈正相关(r=0.354,P<0.05)。结论:4EBP1和P70S6K在宫颈癌发生、发展中起促进作用,二者可作为宫颈癌诊断及靶向治疗的重要生物学标记物。
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| 关 键 词： | 宫颈癌 4EBP1 P70S6K RT-PCR 免疫组织化学 |
The expression and clinical significance of 4EBP1 and P70S6K in different cervical lesions |
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| Abstract: | Objective:To evaluate the expression of 4EBP1 and P70S6K in different cervical lesions and the functions of 4EBP1 and P70S6K in the progression of cervical cancer.Methods:The expressions of 4EBP1 and P70S6K in normal cervix uteri,cervical intraepithelial neoplasia and cervical cancer were detected by the immunohistochemistry(SP methods) and RT-PCR.Results:In normal cervix uteri,cervical intraepithelial neoplasia and cervical carcinomas,both mRNA and protein of 4EBP1 and P70S6K were successively increased(P<0.05).There was a positive correlation between 4EBP1 and P70S6K expression in cervical cancer(r=0.354,P<0.05).Conclusion:High expressions of 4EBP1 and P70S6K may play a promoting role in the pathogenesis and development of cervical cancer.Both of them can be used as important biological markers in diagnosis and targeted therapy of cervical cancer. |
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| Keywords: | Cervical cancer 4EBP1 P70S6K RT-PCR Immunohistochemistry |
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