基质金属蛋白酶-9表达与早产胎膜早破的相关性研究

目的:研究基质金属蛋白酶-9(MMP-9)在胎膜组织上的表达情况与早产胎膜早破的相关性。方法:荧光定量PCR(SYBR Green法)检测MMP-9mRNA;Western blot法检测MMP-9蛋白表达量;结果:早产胎膜早破组与足月胎膜早破组相比较,MMP9不存在统计学差异(Z=-1.783,P=0.075);与自发性早产组比较,MMP9不存在统计学差异(Z=-0.157,P=0.875);而早产胎膜早破组与正常足月产组比较MMP9存在统计学差异(Z=-3.316,P=0.001)。早产胎膜早破组MMP-9蛋白表达高于足月产组,之间的差异具有统计学意义(t=-12.339,P<0.01)。结论:MMP-9与早产胎膜早破存在相关性,在其发病机制中有着重要作用。

Study on correlation between between matrix metalloproteinase-9 expression and preterm premature rupture of membranes

Objective:To study the expression of matrix metalloproteinase-9(MMP-9) in fetal membrane and its correlation with preterm premature rupture of membranes(PPROM).Methods:Fluorescence quantitative PCR(SYBR Green method) was used to detect MMP-9mRNA;Western blot was used to detect MMP-9 protein expression.Results:There was no statistically significant difference in MMP-9 expression between PPROM group and term premature rupture of membranes(TPROM) group(Z=-1.783,P=0.075);there was no statistically significant difference in MMP-9 expression between PPROM group and spontaneous premature delivery group(Z=-0.157,P=0.875);there was statistically significant difference in MMP-9 expression between PPROM group and normal term delivery group(Z=-3.316,P=0.001).The expression level of MMP-9 protein in PPROM group was statistically significantly higher than that in TPROM group(t=-12.339,P<0.01).Conclusion:There is a correlation between MMP-9 and PPROM,MMP-9 plays an important role in pathogenesis of PPROM.