生殖细胞核因子GCNF在宫颈癌中的表达

目的:探讨生殖细胞核因子(GCNF)在宫颈癌中的表达及其在宫颈癌发生发展的作用。方法:运用免疫组织化学Envision法和RT-PCR技术检测GCNF蛋白和mRNA在正常宫颈组织、上皮内瘤变组织及宫颈鳞癌组织中的表达。结果:GCNF蛋白在正常宫颈组织、上皮内瘤变组织(CINⅠ、CINⅡ、CINⅢ)和宫颈鳞癌组织中的表达率分别为90.0%(18/20)、78.3%(18/23)、45.0%(9/20)、33.3%(9/27)和3.4%(4/29),差异有统计学意义(P<0.05)。RT-PCR结果显示GC-NF mRNA的表达与GCNF蛋白相一致,即宫颈鳞癌组织与正常宫颈组织相比GCNF mRNA的表达显著降低(P<0.05)。结论:初步认为GCNF蛋白和mRNA表达的下调或缺失可能是宫颈癌发生的一个早期信号,以期为宫颈癌的靶向治疗提供研究方向。

Expression of germ cell nuclear factor in cervical cancer

Objective:To explore the expression of germ cell nuclear factor(GCNF) in cervical cancer and its role in oncogenesis and development of cervical cancer.Methods:The expressions of GCNF protein and GCNF mRNA were detected by immunohistochemical Envision method and RT-PCR technique in normal cervical tissue,cervical intraepithelial neoplasia(CIN) tissue(CINⅠ,CINⅡ,CINⅢ),and cervical cancer tissue.Results:The expression of GCNF protein in normal cervical tissue,CIN and cervical cancer were 90.0%(18/20),78.3%(18/23),45.0%(9/20),33.3%(9/27),and 3.4%(4/29),respectively,there was statistically significant difference(P<0.05).The results of RT-PCR showed the expression of GCNF mRNA was consistent with GCNF protein,the expression of GCNF mRNA in cervical cancer was statistically significantly lower than that in normal cervical tissue.Conclusion:Preliminary view is that the down-regulation or deletion of expressions of GCNF protein and mRNA may be an early signal of cervical cancer.It may provide research direction for targeted therapy of cervical cancer.