P16蛋白在胃癌组织中的异常表达及其意义

目的：通过研究P16蛋白表达与胃癌相关临床指标的关系，探讨P16蛋白对胃癌发生和发展的影响。方法：应用免疫组化S-P法检测10例正常胃黏膜组织和63例胃癌组织中P16蛋白的表达情况。结果：①正常胃黏膜组织P16蛋白阳性表达率为80.0%，胃癌组织阳性表达率为44.4%，二者之间差异有显著性(P<0.05)；②位于贲门部、胃底体部和幽门部的胃癌P16蛋白阳性表达率之间差异无显著性(P>0.05)；③高分化组P16蛋白阳性表达率明显高于中低分化组(P<0.05)；④癌组织侵犯肌层组P16蛋白阳性表达率低于未侵犯肌层组(P<0.05)；⑤有淋巴结转移组P16蛋白阳性表达率低于无淋巴结转移组(P<0.05)。结论：P16蛋白表达缺失与胃癌之间存在密切关系，暴露于不同侵袭因素作用下所形成的胃癌中P16的表达缺失无差异。

Abnormal expression of P16 protein in gastric carcinoma tissues and its significance

Objective To study the relations between the expression of P16 in gastric carcinoma(GC) tissues and the clinical characteristics of GC,then discuss the effect of P16 on genesis and development of GC.(Methods Expressions) of P16 were examined by S-P immunohistochemica1 staining in 10 cases of normal gastric tissue(NGT) and 63 cases of GC.Results ①The positive rate of P16 expression in NGT was 80.0%,the positive rate in GC was 44.4%.There was significant difference between them(P<0.05).②There were no significant differences of the positive rate of p16 expression between cardiac part,fundus and body of stomach,and pyloric part(P>0.05).③There was significant difference of the positive rate of P16 expression between cases with high differentiation and moderate-low differentiation gastric carcinoma(P<0.05).④Between cases with invasion through muscle and not,there was significant difference(P<0.05).⑤Significant difference could be found between cases with lymph node metastasis and not(P<0.05). Conclusion There are closely relations between the expression loss of P16 and gastric carcinoma.The P16 expression imperfection is indiscriminate in GC which developed under different invasion factors.