| 狼疮鼠淋巴细胞IgG2aV_H区反义RNA重组体的构建及鉴定 |
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| 引用本文： | 邓军卫 颜兰香 陆前进 胡亭亭 佘鸥 夏昆. 狼疮鼠淋巴细胞IgG2aV_H区反义RNA重组体的构建及鉴定[J]. 中南大学学报(医学版), 2001, 26(2): 115-117 |
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| 作者姓名： | 邓军卫 颜兰香 陆前进 胡亭亭 佘鸥 夏昆 |
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| 作者单位： | 邓军卫（中南大学湘雅二医院医学实验中心，长沙410011）;颜兰香（中南大学湘雅二医院皮肤科，长沙 410011）;陆前进（中南大学湘雅二医院皮肤科，长沙 410011）;胡亭亭（中南大学湘雅二医院皮肤科，长沙 410011）;佘鸥（中南大学湘雅二医院医学实验中心，长沙 410011）;夏昆（中南大学湘雅二医院医学实验中心，长沙 410011） |
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| 基金项目： | 国家自然科学基金资助（39770686）。 |
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| 摘 要： | 目的 :构建系统性红斑狼疮 (SLE)鼠模型 (NZW×NZB)F1淋巴细胞IgG2aVH 区反义RNA表达重组体 ,为SLE基因治疗提供基础。方法 :采用逆转录—PCR技术克隆狼疮鼠淋巴细胞IgG2aVH 区cDNA ,经T/A克隆再定向插入表达载体pcDNA3.1,并经酶切电泳 ,PCR ,DNA测序鉴定分析。结果 :经鉴定 ,证明成功构建了表达狼疮鼠淋巴细胞IgG2aVH 区的重组体。结论 :BWF1和鼠淋巴细胞中存在IgG2aVH 区 ,其表达型重组体可用于IgG2aVH 的大量复制.
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| 关 键 词： | 淋巴细胞 cDNA克隆 反义RNA 小鼠 |
| 文章编号： | 1000-5625(2001)02-0115-03 |
| 修稿时间： | 2000-11-26 |
Construction ofrecombinant antisense RNA IgG2aVH region of murine systemic lupus erythematous |
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| DENG Jun-wei. Construction ofrecombinant antisense RNA IgG2aVH region of murine systemic lupus erythematous[J]. Journal of Central South University. Medical sciences, 2001, 26(2): 115-117 |
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| Authors: | DENG Jun-wei |
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| Affiliation: | (Center Research Laboratory, The Second Xiangya Hospital, Central South University, Changsha, 410011, China) |
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| Abstract: | Objective To construct recombinant plasmid of IgG2aV H region antisense RNA of systemic lupus erythematous. Methods Total RNA was isolated from spleen cell of BWF1 mice. Using spleen cell total RNA as the template, We designed specific primers from A6.1 region sequences, and amplified the IgG2aV H region 375 bp DNA fragments by RT PCR. The IgG2aV H cDNA was cloned by T/A and inserted into pcDNA 3.1 plasmid of vector. Results The IgG2aV H antisense RNA plasmid expressing recombinant was identified by restriction enzyme digestion and DNA sequence analysis. Conclusion There is IgG2aV H gene in F1 mice spleen cell; the IgG2aV H antisense RNA expressing recombinants is constructed successfully. |
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| Keywords: | lymphocytes； cDNA clone； antisense RNA； mice； |
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