神经干细胞在胚胎脑皮质发育过程中的变化

目的 比较3个不同胚胎时期神经干细胞的增生、分化和凋亡等生物学性状,了解胚胎脑皮质发育过程.方法E14、 E18和E20胎鼠前脑脑皮质来源的神经干细胞在原代培养后,用BrdU细胞增生检测试剂盒检测增生能力;诱导分化7天后,对细胞分别进行MAP2和GFAP免疫荧光标记和Hoechst 33342核染色,计算MAP2 细胞和GFAP 细胞占Hoechst 细胞的各自比例;用Annexin V-FITC凋亡试剂盒检测早期凋亡率.结果 3组神经干细胞的OD值分别是:E14为1.1771±0.0422(n=22),E18为0.4127±0.0328(n=23),E20为0.2117±0.0456(n=16)(均为P<0.001);E14神经元细胞比例和星形胶质细胞比例分别为:81.96%±4.91%(n=10)和16.63%±4.05%(n=10),E18为27.56%±3.54%(n=10)和58.98%±5.91%(n=10),E20为4.75%±2.39%(n=10)和89.43%±3.40%(n=10)(均为P<0.001);早期凋亡率分别为:E14为41.12%±9.75%(n=8),E18为56.30%±9.35%(n=19),E20为5.74%±3.31%(n=9)(均为P<0.01).结论神经干细胞的增生能力随着胚胎发育变得越来越弱,而凋亡比例先小后大,最后逐渐变小.神经干细胞分化为神经元细胞的比例随着胚胎发育过程会越来越小,而星形胶质细胞的比例会越来越大.

Comparing the Proliferation, Differentiation and Apoptosis of the Neural Stem Cells from Embryonic Brains of Different Stages

Objective With the comparison of the proliferation,differentiation and apoptosis of neural stem cells from different embryonic stages,to obtain the biology of NSCs and the development mechanism of embryonic cortex underlining.Methods After the prime culture of NSCs from forebrain cortice of different embryonic stages(E14,E18 and E20),enzyme immunoassay for NSCs proliferation was carried out.After 7 days of differentiation induced by fetal bovine serum,they were detected by the neuronal marker MAP2 and the a...