| 人胃癌腹膜高转移潜能细胞系的基因表达分析 |
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| 引用本文： | 白飞虎,杨力,王钧,惠亮亮,苗雨,吴开春,樊代明. 人胃癌腹膜高转移潜能细胞系的基因表达分析[J]. 中华肿瘤防治杂志, 2010, 17(5) |
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| 作者姓名： | 白飞虎 杨力 王钧 惠亮亮 苗雨 吴开春 樊代明 |
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| 作者单位： | 1. 宁夏医学院附属医院消化内科,宁夏,银川,750004
2. 第四军医大学西京医院全军消化病研究所肿瘤生物学国家重点实验室,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金(30160033,30860332); 部分资金来源于教育部春晖计划(Z2006-1-75001); 宁夏回族自治区卫生厅重点项目(2008); 宁夏回族自治区教育厅重点项目(2008) |
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| 摘 要： | 目的:比较人胃癌细胞系与其腹膜高转移潜能细胞系的基因表达谱,寻找与胃癌腹膜转移相关的基因表达改变。方法:采用基因芯片技术,比较遗传背景相同但转移力有明显差异的两个细胞系GC9811和GC9811-P,分析其基因表达谱的差异。选取部分基因行RT-PCR进一步验证基因芯片的准确性。结果:在11901个候选基因中,筛选出248个(2.1%)差异表达基因。GC9811-P与GC9811相比,表达上调的基因有218个,下调的有30个,包括DNA合成和错配修复基因如H3F3A、细胞增生基因、蛋白合成与修饰基因、信号传导基因和离子通道与运输蛋白相关基因等。半定量RT-PCR对PTEN、S100A4和ZNRD1基因检测结果验证了基因芯片数据的可靠性。结论:胃癌腹膜转移是多基因作用的综合结果,筛选的基因对预测胃癌腹膜转移和抗转移干预措施可能有指导意义。
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| 关 键 词： | 细胞系 胃肿瘤 腹膜转移 差异表达基因 基因芯片 |
Differential gene expression profiles of gastric cancer cells with high metastatic potential to peritoneum |
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| BAI Fei-hu,YANG Li,WANG Jun,HUI Liang-liang,MIAO Yu,WU Kai-chun,FAN Dai-ming. Differential gene expression profiles of gastric cancer cells with high metastatic potential to peritoneum[J]. Chinese Journal of Cancer Prevention and Treatment, 2010, 17(5) |
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| Authors: | BAI Fei-hu YANG Li WANG Jun HUI Liang-liang MIAO Yu WU Kai-chun FAN Dai-ming |
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| Affiliation: | BAI+Fei-hu1,YANG+Li1,WANG+Jun2,HUI+Liang-liang1,MIAO+Yu1,WU+Kai-chun2,FAN+Dai-ming2+1.Department+of+Digestion,Affiliated+Hospital+of+Ningxia+Medical+College,Yinchuan+750004,P.R.China2.State+Key+Laboratory+of+Cancer+Biology+%26+Institute+of+Digestive+Diseases,Xijing+Hospital,Fourth+Military+Medical+University,Xi%5c'an+710032,P.R.China |
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| Abstract: | OBJECTIVE:To study gene expression profile of human gastric carcinoma cell line with peritoneal metastatic potential for screening peritoneal metastasis-related genes.METHODS:The global analysis was performed on differential gene expression of a gastric cancer cell line(GC9811)and its derivative sublines with high potential for metastasis to the peritoneal cavity(GC9811-P).By applying a high-density oligonucleotide array method,the expressions of approximately 11 901 genes were analyzed,and some selected ge... |
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| Keywords: | cell line stomach neoplasms peritoneal metastasis differential gene expression cDNA microarray |
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