抗双链DNA抗体不同检测方法的比对分析

目的评价间接免疫荧光法(IIFA)、酶联免疫吸附测定法(ELISA)和免疫印迹法(IBT)检测抗双链DNA(dsDNA)抗体的特点及应用价值。方法采用IIFA、ELISA和IBT法对实验组(系统性红斑狼疮患者,n=70)及健康对照组(健康人,n=650)血清标本进行抗dsDNA抗体的检测。结果经IIFA、ELISA法及IBT法检测,实验组抗dsDNA抗体的阳性率分别为34.29%,54.29%和44.29%;健康对照组抗dsDNA抗体的阳性率分别为0.15%,4.46%和1.54%,ELISA和IBT检出阳性率与IIFA方法比较,差异有统计学意义(P<0.05)。结论在血清抗dsDNA抗体的检测上,IIFA法特异性高,敏感性较低;而ELISA和IBT法检测敏感性高,特异性较差。

Comparative analysis of different detection methods for anti-double stranded DNA antibody

Objective To evaluate the characteristics and application value of anti-double stranded DNA(dsDNA) antibody detection by indirect immunofluorescent assay(IIFA),enzyme-linked immunosorbent assay(ELISA) and immunoblotting test(IBT).Methods IIFA,ELISA and IBT methods were employed to detect anti-dsDNA antibody in serum sample of experimental group(patients with systemic lupus erythematosus,n=70) and healthy control group(healthy people,n=650).Results Detection by IIFA,ELISA and IBT methods,positive rates of anti-dsDNA antibody in experimental group were 34.29%,54.29% and 44.29%,respectively,however,the positive rates of that in the healthy control group were 0.15%,4.46% and 1.54%,respectively.Compared the positive rate detected by IIFA with which detected by ELISA or by IBT methods,there were statistically significant differences(P<0.05).Conclusion Detection of serum anti-dsDNA antibody,IIFA has higher specificity and lower sensitivity,while ELISA and IBT have higher sensitivity and lower specificity.