白细胞介素-17及其受体在鼻息肉组织中的表达及意义

目的 检测鼻息肉组织中白细胞介素（interleukin，IL）-17及其受体的表达，并探讨其在鼻息肉免疫发病机制中的作用。方法 对20例患者鼻息肉组织及15例对照组织（接受鼻中隔偏曲矫正术的患者）分别进行IL-17及其受体免疫组织化学染色和免疫印迹检测。结果 免疫组化检查发现IL-17主要表达在鼻息肉组织中的浆细胞胞质内，少量表达在上皮细胞棘细胞层和浆液腺泡内，与对照组的表达比较差异有统计学意义（t值分别为2．237、2．176、2．246，P值均〈0．05）。而且免疫印迹检查发现IL-17及其受体蛋白在鼻息肉组织中特异性表达强度高于对照组。结论 IL-17通过与其受体特异性结合，在鼻息肉组织中表达增高，对其发病起着一定的作用。

Expression and significance of interleukin-17 and its receptor in nasal polyps

Objective To detect the expression and distribution of interleukin-17 and interleukin 17 receptor (IL-17R) in nasal polyps and to probe into their significance in the pathology of nasal polyps. Methods The methods of immunohistochemical staining and western blot were used to detect IL-17 and IL-17R in nasal polyps and controls. Results In nasal polyp tissues, IL-17 expressed mainly in cytoplasm of plasm cell,less in prickle-cell layer of the epithelium and the acinus of the serous gland. In turbinates, IL-17 also expressed in the cytoplasm of the plasm cell, the prickle-cell layer of the epithelium and the acinus of the serous gland. The expression of IL-17 between nasal polyps and turbinates differed significantly (t=2.237,2.176,2.246,P<0.05,respectively). Both IL-17 and IL-17R displayed specific bands in nasal polyps and turbinates, but the bands of IL-17 and IL-17R in nasal polyps!were stronger than those in turbinates. Conclusions IL-17 may have an important role in the occurrence of nasal polyps by specific combination with IL-17R and over-expression in nasal polyps.