| 高效液相色谱法测定复方枣仁胶囊中左旋延胡索乙素含量 |
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| 引用本文： | 池秀珍.高效液相色谱法测定复方枣仁胶囊中左旋延胡索乙素含量[J].中国药业,2009,18(10):40-41. |
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| 作者姓名： | 池秀珍 |
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| 作者单位： | 福建省泉州市药品检验所,福建,泉州,362000 |
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| 摘 要： | 目的建立测定复方枣仁胶囊中左旋延胡索乙素含量的高效液相色谱（HPLC）法。方法采用Discovery C18（150mm×4．6mm，5／xm），以甲醇-0．1％磷酸（63：37，pH=6．0）为流动相，检测波长为281nm，流速为1．0mL／min，柱温为30℃。结果左旋延胡索乙素质量浓度在63．52～635．2μg／mL范围内与峰面积线性关系良好，r=0．99998（n=7），平均回收率为99．93％，RSD为0．7％（n=6）。结论HPLC法灵敏、快捷、准确，可用于复方枣仁胶囊中左旋延胡索乙素含量的测定。
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| 关 键 词： | 复方枣仁胶囊 左旋延胡索乙素 高效液相色谱法 含量 |
Determining of L- Tetrahydropalmatine in Compound Zaoren Capsules by HPLC |
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| Chi Xiuzhen.Determining of L- Tetrahydropalmatine in Compound Zaoren Capsules by HPLC[J].China Pharmaceuticals,2009,18(10):40-41. |
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| Authors: | Chi Xiuzhen |
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| Institution: | Chi Xiuzhen (Quanzhou Institute for Drug Control, Quanzhou, Fujian, China 362000) |
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| Abstract: | Objective To establish a HPLC method for determining the content of L- tetrahydropalmatine in Compound Zaoren Capsules. Methods The Discovery C18 column（150 mm×4.6 mm, 5 μm） with methanol- 0. 1% phosphoric acid （63 ： 37, pH = 6.0） as the mobile phase was used. The flow rate was 1.0 mL/min and the detective wavelength was 281 nm. The column temperature was 30℃. Results Good linearity of L- tetrahydropahnatine was obtained within the range 63.52- 635.2 μg/mL. The average recovery rate was 99.93% and RSD was 0.7% （n =6）, r =0. 999 98（n = 7）. Conclusion The method is accurate and reliable with a high sensitivity, which can be used for determining the content of L- tetrahydropalmatine in Compound Zaoren Capsules. |
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| Keywords: | Compound Zaoren Capsules L - tetrahydropalmatine HPLC content |
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