Determination of Sertoli cell numbers in the developing rat testis by stereological methods |
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Authors: | Z. X. WANG N. G. M. WREFORD D. M. DE KRETSER |
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Affiliation: | Department of Anatomy, Monash University, Melbourne, Victoria, Australia. |
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Abstract: | Stereological studies were performed to determine the number of Sertoli cells present during the postnatal development of the rat testes. Sprague-Dawley rats aged from 1 to 70 days were used in two experiments, and in each were fixed by vascular perfusion and embedded in Epon-Araldite, subsequent to which 1 micron sections stained with Toluidine blue were prepared. In the first experiment, rats aged from 1 to 20 days were used in groups of three, and number estimates were made using a direct counting method. In the second, which used groups of four rats aged from 20 to 70 days, a point sampled intercept was used to estimate nuclear volume and thence number. The results of the experiments indicate that the newborn rat testis contains 1.3 +/- 0.2 x 10(6) Sertoli cells and that this number increases to 38.4 +/- 2.7 x 10(6) at day 15. No further increase in Sertoli cell number occurred thereafter up to day 70 of age. |
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Keywords: | Sertoli cell morphometry FSH testis |
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