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First trimester chorionic villi sampling and direct chromosome preparations |
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| Authors: | A T Tharapel J V Dacus S A Tharapel J Dempsey G Flinn D C Shaver D Massouda R Sid Wilroy Jr |
| Institution: | Divison of Genetics, Department of Pediatrics, University of Tennessee, Memphis - The Health Science Center, Memphis, Tennessee;Maternal and Fetal Division, Department of Obstetrics and Gynecology, University of Tennessee, Memphis - The Health Science Center, Memphis, Tennessee;Diagnostic Ultrasound consultants, Memphis, Tennessee, USA |
| Abstract: | Chorionic villi sampling was performed on 52 patients prior to elective termination of their pregnancies. Villi were obtained in 42, and direct chromosome preparations were successful in 41 of them. The use of a mixture of 0.075 M potassium chloride and 1% sodium citrate in the ratio of 2:1 for hypotonic treatment and 40% acetic acid for cell dispersal yielded chromosomes with good morphology and G-bands. |
| Keywords: | Chorionic villi prenatal diagnosis |