| 光亲和标记测定烫伤大鼠肝胞液糖皮质激素受体分子量 |
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| 引用本文： | 杨义力,谭金兴,徐仁宝. 光亲和标记测定烫伤大鼠肝胞液糖皮质激素受体分子量[J]. 第二军医大学学报, 1987, 0(4) |
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| 作者姓名： | 杨义力 谭金兴 徐仁宝 |
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| 作者单位： | 第二军医大学病理生理学教研室(杨义力，谭金兴)，第二军医大学病理生理学教研室(徐仁宝) |
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| 摘 要： | 本实验室以往的工作证明,大鼠烫伤后,不但有肝胞液糖皮质激素受体结合容量的改变,而且有表观解离常数的增大和糖皮质激素受体沉降系数的改变。本文采用[~3H]去炎松缩酮光亲和标记法测定了烫伤大鼠肝胞液糖皮质激素受体的分子量,和正常大鼠无显著差异,因而说明,受体沉降系数的改变不是由于受体结合亚单位分子量的改变所致。
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| 关 键 词： | 光亲和标记 糖皮质激素受体 烫伤 |
DETERMINATION OF THE MOLECULAR WEIGHT OF PHOTOAFFINiTY LABELED GLUCOCORTICOSD RECEPTOR IN SCALDED RAT LIVER CYTOSOL |
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| Yang Yili,et al. DETERMINATION OF THE MOLECULAR WEIGHT OF PHOTOAFFINiTY LABELED GLUCOCORTICOSD RECEPTOR IN SCALDED RAT LIVER CYTOSOL[J]. Former Academic Journal of Second Military Medical University, 1987, 0(4) |
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| Authors: | Yang Yili et al |
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| Affiliation: | Department of Pathophysiology |
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| Abstract: | Previous work in our laboratory showed that, after scalding, there was not only a decrease of rat liver cytosol [3H]-Dexamethasone specific binding sites but also an increase of apparent dissociation constant of [3H]-Dex and a change of sedimentation coefficient of glucocorticoid receptor (GCR).The present study was carried out using photoaffinity labeling of GCR in rat liver cytosol.It was found that there was no significant difference between molecular weights of . GCR in normal and scalded rats determined by SDS-polyacrylamide gel electrophoresis. Our result suggests that change of sedimentation coefficient is not due to alteration of molecular weight of GCR binding subunit. |
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| Keywords: | photoaffinity labeling glucocorticoid receptor scalding |
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