亚硒酸钠拮抗氯化镉染毒大鼠肝、肾细胞损伤的研究

目的观察亚硒酸钠处理对亚慢性氯化镉中毒大鼠肝、肾结构的影响,为氯化镉慢性中毒的防治和亚硒酸钠蓄积性毒性研究提供依据。方法雄性SD大鼠54只,随机分成3组。对照组大鼠饮用蒸馏水;其余两组大鼠饮用含30 mg/L氯化镉蒸馏水;硒干预组大鼠给予5μmol/kg亚硒酸钠溶液灌胃。结果与对照组比较,氯化镉组大鼠组织中GSH-Px及SOD活性降低、MDA含量增加;硒干预组大鼠组织GSH-Px及SOD活性显著升高、MDA含量降低。组织病理切片观察结果,单纯给氯化镉组大鼠肾小球肿胀或固缩,肾小囊囊腔增大,肾小管上皮细胞变性坏死并脱落等病理损伤。硒干预组大鼠肝、肾脏组织结构未见明显损伤。结论饮用含氯化镉的水源可致肾脏组织明显损伤,低剂量亚硒酸钠可拮抗氯化镉对肾组织的损伤作用,对氯化镉所致组织氧化损伤有一定的修复作用。

Protective Effects of Sodium Selenite against Cadmium Chloride-Induced Injury on Kidney and Liver Cells of Rat

Objective To study the-induced injury on kidney and liver cells of rat and the protective effects of sodium selenite.Methods 54 healthy SPF male SD rats were divided randomly into three groups.GroupⅠ:rats drinking distilled water as control;GroupⅡ:rats drinking water containing 30mg/L CdCl2distilled water;Group Ⅲ:rats drinking water containing 30mg/L CdCl2 distilled water,while giving 5μmol/kg Na2SeO3solution gavage,5 days a week,once a day.Rats were killed on the 2nd,4th and 8th week after initiation of the experiment.Blood were collected,kidneys and livers were removed immediately,?xed in 10% methanal ?xative,routinely processed and stained with hematoxylin and eosin.Results In Se-administered rat homogenates,the contents of MDA were lower,and the activity of GSH-Px and SOD were significantly higher than those of control.In Cd-treated rats,we noticed histopathological changes in kidneys and livers,while no obvious changes were found in Se-administered rats.Conclusion The sodium selenite could efficiently protect the-induced injury on kidney and liver cells of rat.