姜黄素醇质体的制备及其质量评价

目的:制备姜黄素醇质体,并考察其理化性质.方法:采用乙醇注入法制备姜黄素醇质体,以包封率为考察指标,采用正交试验法优选处方,并用透射电镜观察其形态,激光粒度仪测定粒径和Zeta电位,以超速离心法分离含药醇质体与游离药物,用HPLC法测定姜黄素醇质体的包封率.结果:优选处方为:姜黄素10 mg、蛋黄卵磷脂350 mg、胆固醇50 mg、乙醇百分浓度25%.实验所制醇质体纳米粒子为类球形囊泡结构,粒径为(210.8±2.0)nm,Zeta电位为(-3.49±0.27)mV,粒径分布均匀,多分散指数(PDI)为(0.144±0.006),以优选后处方制备醇质体,其包封率为(85.55±2.12)%.结论:乙醇注入法适用于姜黄素醇质体的制备,所制醇质体纳米粒子各项物理指标稳定,可用于经皮渗透给药的研究.

Preparation and Quality Evaluation of Curcumin Ethosomes

Objective: To prepare curcumin ethosomes and investigate the physicochemical properties.Method: Curcumin ethosomes were prepared by ethanol injection method.The formulation was optimized by orthogonal test with encapsulation efficiency as the index.The morphology was examined by transmission electron microscope.The particle size and Zeta potential were determined by laser granularity equipment.The free drug was separated by ultracentrifugation method and the encapsulation efficiency of the ethosomes was determined by HPLC.Result: The optimized formulation was as follows: the content of curcumin,egg phophatidylcholine and cholesterol and the percent concentration of ethanol was 10 mg,350 mg,50 mg and 25%,respectively.The curcumin ethosomes were spherical vesicles.The size distribution was narrow with polydispersity index(PDI) of 0.144±0.006.The particle diameter,Zeta potential and encapsulation efficiency was(210.8±2.0) nm,(-3.49±0.27) mV and(85.55±2.12)%,respectively.Conclusion: The ethanol injection method is appropriate for the preparation of curcumin ethosomes with stable physical properties,which may be used in transdermal drug delivery systems.