Bax/Bcl-2在淋巴回流障碍大鼠肾组织中的表达

目的：探讨阻断肾淋巴循环对大鼠肾脏细胞Bax、Bcl-2表达的影响及与大鼠肾脏功能的关系。方法选取雄性Wistar大鼠48只,将其随机分为模型组和对照组,各24只。各组大鼠分别于术后第1、7、14、28天各处死6只,留取肾组织标本提取组织蛋白、mRNA和制作石蜡切片。运用Real-time PCR、Western blot和免疫组织化学检测Bax、Bcl-2在肾组织中的表达,并测定24 h尿蛋白和血肌酐水平。结果模型组大鼠的肾功能逐渐减退,随着术后时间的延长,肾功能损害逐渐加重。模型组大鼠的Bax表达明显强于对照组,免疫组织化学显示,Bax的表达主要在肾小管及肾间质,远端小管的表达尤其明显,相反,模型组大鼠的Bcl-2的表达明显减弱。结论阻断肾淋巴循环可导致大鼠肾功能及肾小管间质的损害,并随时间延长而加重,肾细胞凋亡与此密切相关,其中Bax/Bcl-2途径发挥了积极作用。

Expression of Bax/Bcl-2 in renal tissue of rats with lymphatic flow barrier

Objective To investigate the influence of blocking renal lymph circulation on the expression of Bax/Bcl-2 in kidney cells of rats and the relationship of between the expression of Bax/Bcl-2 and kidney function of rats. Meth-ods 48 male Wistar rats were randomly divided into the model group （n=24） and the Control group （n=24）.6 rats in each group were put to death after 1,7.14,28 days reapectively,and nephridial tissue sample were obtained for extracting protein,mRNA and making paraffin section.The expression of Bax/Bcl-2 in renal tissue was tested using Real-time PCR,Western blot and immunohistochemistry.24 hours urine protein and serum creatinine level were determined. Re-sults The renal function of rats in the model group decreased gradually,and with prolonging of postoperative time,renal function injury aggravated gradually.The expression of Bax of rats in the model group was stronger than that of the con-trol group,immunohistochemistry showed that Bax mainly expressed in renal tubule and interstitium,expression of the distal tubule is particularly evident,on the contrary,the expression of Bcl-2 of rats in the model group decreased obvi-ously. Conclusion Blocking renal lymph circulation can lead to the damage of renal function and renal tubular intersti-tial,and with prolonging of time,the damage aggravated gradually,renal cell apoptosis is closely related to that,among them,Bax/Bcl-2 pathway plays a positive role.