微生态制剂对实验性大鼠结肠炎的治疗

目的 评价微生态制剂双歧三联活菌对三硝基苯磺酸钠(TNBS)诱导的大鼠结肠炎的疗效,探索炎症性肠病(IBD)治疗的新方法.方法 成年雌性SD大鼠50只,随机分为对照组(G1)、模型组(G2)、双歧三联活菌治疗组(G3)、奥沙拉秦治疗组(G4)、双歧三联活菌和奥沙拉秦联合治疗组(G5),每组10只.ELISA法检测各组的血清C反应蛋白(CRP)、TNFα、IL-10水平,分光光度法检测肠组织髓过氧化物酶(MPO)活力,并对肠组织进行病理组织学分析.结果 治疗后,G1组肠组织结构正常,血清CRP、TNFα、IL-10水平、结肠黏膜损伤指数(CMDI)及肠组织MPO活力显著低于G2组(P<0.001);G2组肠组织炎症程度最莺,血清CRP、TNFα、IL-10水平、CMDI及肠组织MPO活力最高,P<0.05;3个治疗组G3、G4、G5组的肠组织炎症呈不同程度消散,血清CRP、TNFα、IL-10水平及肠组织MPO活力呈不同程度下降,以G5组最显著,P<0.05;G2组血清CRP、TNFα、IL-10及肠组织MPO活力均分别与CMDI呈正相关,P<0.001.结论 双歧三联活菌能有效改善TNBS诱导的大鼠结肠炎,其机制可能与调节细胞因子水平有关.

Probiotic therapy using live combined bifidobacterium, lactobacillus and enterococcus for experimental colitis in rats model

Objective To evaluate the effect of live combined bifidobacterium, lactobacillus and enterococcus capsules for colitis in rats induced by trinitrobenzenesulfonic acid (TNBS), so as to explore a new therapy for inflammatory bowel diseases (IBD). Methods 50 female adult Sprague-Dawley rats were randomly divided into 5 groups i. e. normal control group(G1) ,untreated TNBS-induced colitis(G2) ,TNBS-induced colitis treated with live combined bifidobacterium, lactobacillus and enterococcus (G3), TNBS-induced colitis treated with olsalazine (G4) and TNBS-induced colitis treated with both live combined bifidobacterium, lactobacillus and enterococcus and olsalazine at the same dose and duration (G5). Each group received its respective treatment. Serum levels of C-reactive protein (CRP), TNFα and IL-10 were measured with ELISA, colonic myeloperoxidase (MPO) activity was determined with spectrophotometric method, histopathologic picture of the colon of each rat was studied with microscope and colonic mucosa damage index(CMDI) was recorded. Results Serum CRP,TNFα,IL-10,CMDI and colonic MPO in G1 were significantly lower than those in G2 (P < 0. 001) with normal colonic architecture. G2 exhibited the most severe colonic inflammation and the highest levels of CRP,TNFα, IL-10, CMDI and colonic MPO with stastical significance. Treatment groups G3, G4 and G5 showed more obvious colonic inflammatory remission and lower levels of serum CRP,TNFα , IL-10 and colonic MPO, G5 being most notable when compared to G2 with stastical significance. In G2, serum levels of CRP, TNFα, IL-10 and colonic MPO activity each correlated positively with CMDI (P < 0. 001). Conclusions Live combined bifidobacterium, lactobacillus and enterococcus can effectively ameliorate colitis in rats induced by TNBS; the underlying mechanism may possibly be associated with the serum levels of cytokines.