Preparation and characterization of murine monoclonal antibodies toSalmonella typhimurium K-antigen |
| |
| Authors: | M. V. Raevskaya Yu. A. Belaya N. V. Koval'chuk L. N. Chernousova V. G. Petrukhin |
| |
| Affiliation: | (1) Department of Cell Physiology and Immunology, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow;(2) N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow;(3) Institute of Tuberculosis, Moscow |
| |
| Abstract: | Monoclonal antibodies toS. typhimurium K-antigens representing IgG1 immunoglobulins with a “kappa”-type light chain are prepared and characterized. They are highly active in indirect enzyme immunoassay with purified K-antigens and whole fixedS. typhimurium cells and virtually do not cross-react with O-antigen of the same bacterial species or withE. coli antigenic structures. The kinetics of binding and ability to agglutinate whole bacterial cells are studied and epitope analysis is carried out, which shows that antigenic determinants ofS. typhimurium K-antigen qualitatively differ from those of O-antigen of the same bacterium. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N o 6, pp. 633–635, June, 1994 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences |
| |
| Keywords: | monoclonal antibodies Salmonella antigen enzyme immunoassay |
| 本文献已被 SpringerLink 等数据库收录！ |
|
