直接法McAb-Dot-ELISA检测日本血吸虫感染耕牛血清循环抗原的研究

将抗日本血吸虫单克隆抗体标记辣根过氧化物酶,进行直接法单克隆抗体斑点酶联免疫吸附试验(McAb-Dot-ELISA),检测日本血吸虫感染耕牛的血清循环抗原。结果,该方法对实验感染耕牛的阳性检出率为100％(32/32);对安徽、江西和湖南3省血吸虫病流行区自然感染耕牛(粪孵阳性)的阳性检出率分别为93.93％(418/445),88.50％(100/113)和81.71％(143/175);对健康耕牛的阴性符合率为98.51％(66/77),对16份感染锥虫和8份实验感染肝片吸虫的耕牛血清均未见交叉反应。提示,该方法具有操作简便、快速等优点,可作为一种新的耕牛血吸虫病免疫诊断方法在疫区基层推广应用。

DIRECT MCAB-DOT-ELISA FOR DETECTION OF CIRCULATING ANTIGENS IN SERA OF BOVINES INFECTED WITH SCHISTOSOMA JAPONICUM

An antischistosoma monoclonal antibody tabled with horseradish peroxidase was used in direct McAb-Dot-ELISA test for detection of circulating antigens in sera of bovines infected with Schistosoma japonicum. Results showed that the positive rate of 5. japonicum-inoculated bovines was 100% (32/32). while the positive rates of naturally infected bovines in Anhui. Jianxi and Hunan Province were 93. 90% (418/445). 88. 50% (100/113) and 81. 71%(143/ 175) respectively. The negative rate of non-infected bovines was 98. 50% (66/67). There were no cross reactions with Trypanosoma infected (16/16) and Fasciola hepatica-infected (8/ 8) bovines. The study suggested that this method was simple and time-saving, and possessed the potential value as a novel diagnostic method in detecting animal infection in field trials.