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| 芦丁与槲皮素对成骨细胞代谢影响的比较研究 | |
| 引用本文： | 杨亚军,杨中林,王冬春,肖小春,李萍.芦丁与槲皮素对成骨细胞代谢影响的比较研究[J].中药材,2006,29(5):467-470. |
| 作者姓名： | 杨亚军 杨中林 王冬春 肖小春 李萍 |
| 作者单位： | 1. 中国药科大学中医药教研室,江苏,南京,210038 2. 江苏先声药物研究有限公司,江苏,南京,210042 3. 中国药科大学教育部现代中药重点实验室,江苏,南京,210038 |
| 摘 要： | 目的:对比研究芦丁(Rutin)与其苷元槲皮素(Quercetin)对成骨细胞形成的影响,以探讨二者防治骨质疏松的作用机理。方法:新生大鼠颅骨分离培养成骨(ROB)细胞,MTT法测定细胞增殖,比色法测定碱性磷酸酶(ALP)活性,茜素红(AR-S)染色法测定矿化结节数,以观察Rutin与Quercetin对ROB细胞骨形成过程中增殖、分化和矿化作用的影响。结果:Quercetin可明显促进ROB细胞的增殖与矿化,故能增强ROB细胞形成活性;Rutin仅能促进ROB细胞增殖,而对矿化作用无影响,却能抑制ROB细胞分化,因而无明显促进骨形成活性。结论:Querce- tin中3-OH形成糖苷(Rutin)后可引起体外培养的成骨细胞活性降低,故含有Rutin的食物或中药防治骨质疏症的机理可能是通过其体内代谢物Quercetin刺激成骨细胞形成而发挥作用的。 |
| 关 键 词： | 槲皮素 芦丁 成骨细胞 骨质疏松 |
| 文章编号： | 1001-4454(2006)05-0467-04 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005年9月26日 |
Comparative Study on Effects of Rutin and Quercetin on Metabolism in Osteoblast Cells |
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| YANG Ya-jun,YANG Zhong-lin,WANG Dong-chun,XIAO Xiao-chun,LI Ping.Comparative Study on Effects of Rutin and Quercetin on Metabolism in Osteoblast Cells[J].Jorunal of Chinese Medicinal Materials,2006,29(5):467-470. | |
| Authors: | YANG Ya-jun YANG Zhong-lin WANG Dong-chun XIAO Xiao-chun LI Ping |
| Institution: | 1. Department of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China; 2. Jiangsu Simcere Pharmaceutical Research Company Ltd, Nanjing 210042, China; 3. Key Lab for Modem Traditional Chinese Medicine, Ministry of Education, China Pharmaceutical University, Nanjing 210038, China |
| Abstract: | Objective: To investigate the mechanism of rutin and its aglycone (quercetin) in preventing and treating osteoporosis and their effects on stimulating bone formation were studied comparatively in osteoblast cells. Methods: The effects of rutin and quercetin on the proliferation, differentiation and mineralization were studied respectively by using tetrazolium (MTT) , alkaline phosphatase ( ALP) and mineralized nodules assays in newborn rat calvarial osteoblast (ROB) cells. Results: Quercetin showed significant stimulatory effects on proliferation and mineralization in ROB cells, so it could promote bone formation. However, rutin could not imporve stimulation of bone formation because it increased proliferation and inhibited differentation and had no remarkable influence on ROB cells in vitro. Conclusion: Rutin showed less acticvity than quercetin on bone formation in ROB cells in vitro. Therefore the mechanism that some foods and traditional Chinese medicine containing rutin can prevent and treat osteoporosis is that its metaboite in vivo can promote bone formation in ROB cells. |
| Keywords: | Quercetin Rutin Osteoblast Osteoporosis |
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