苦参碱注射液对AD模型大鼠脑组织白介素1β、NO含量及nNOS免疫阳性神经元的影响

为探讨苦参碱注射液对老年性痴呆(AD)模型大鼠脑组织白介素1β(IL-1β)、NO含量及nNOS免疫阳性神经元的影响以及药物的作用效果及机制,本研究用SD大鼠将鹅膏蕈氨酸(IBO)双侧海马立体定位注射造模,实验分为对照组、高剂量苦参碱治疗组、低剂量苦参碱治疗组、模型组和哈伯因组。检测大鼠大脑皮质和海马内IL-1β、NO的含量变化以及nNOS免疫阳性神经元的形态改变。结果显示:模型组大鼠大脑皮质和海马内IL-1β及NO的含量均显著高于对照组(P<0.01);高剂量治疗组大脑皮质和海马内IL-1β及NO的含量均低于模型组(P<0.05);模型组nNOS免疫阳性神经元有明显变性、数目减少;高剂量治疗组大鼠nNOS免疫阳性神经元的数目与模型组比较明显增多(P<0.05),变性有所减轻;但低剂量组的nNOS免疫阳性神经元数与模型组没有显著差异(P>0.05)。本文结果提示,模型组大鼠大脑皮质和海马内IL-1β及NO的含量均显著增高,可能为导致AD神经元损伤的原因之一,苦参碱注射液对AD神经元损伤的保护作用可能是通过下调IL-1β和NO而实现的。

EFFECT OF MATRINE INJECTION ON THE LEVEL OF IL-1β, NO AND Nnos-POSITIVE NEURONS IN THE CENTRAL NERVOUS SYSTEM IN ALZHEIMER'S DISEASE MODEL RATS

To study the effect of Matrine injection on the level of IL-1β, NO and nNOS-positive neurons in the cerebral cortex and hip pocampal formation in Alzheimer’s disease model rats. Ibotenic acid (IBO) was directly injected into hippocampus to make the SD model rats. The animals were divided into 5 groups: control group, high-dose treatment group, low-dose treatment group, model group and Huperzine Atreatment group. For each group, the level’s of IL-1β and NO were assayed with radioimmunoassay and spectrophotometer, respectively, and the changes of nNOS-positive neurons in the frontal cortex and hippocampus were observed with immunohistochemistry. The results showed: (1) The levels of IL-1β and NO in the frontal cortex and hippocampus were markedly increased in model group compare to that in the control group (P<0.01). After matrine injection administration, the levels of IL-1β and NO in the frontal cortex and hippocampus decreased in high-dose treatment group were compared to that in the model group (P<0.05). (2) The number of nNOS-positive neurons also decreased in the model group was compared to that in the control group (P<0.05). But after matrine injection administration, the number of nNOS-positive neurons increased and the pathological changes improved in high-dose treatment group were compared to that in the model group(P<0.05). However, there was no significant change in low-dose treatment group, compared to that in the model group. The levels of IL-1β and NO were increased in the model group, these could be one of the reasons resulting in neuronal damage. The protective effect of matrine injection on neuronal damage could be due to its reducing the production of IL-1β and NO in this model.