nm23H1对肝癌细胞增殖及体内肿瘤形成能力的影响

目的 研究ｎｍ２３Ｈ１对肝癌细胞增殖、体内肿瘤形成和转移的影响。方法 构建正反义ｎｍ２３Ｈ１ ｃＤＮＡ表达载体并转染肝癌细胞ＳＭＭＣ－７７２１，得到ｎｍ２３Ｈ１稳定最高和最低表达的两种细胞克隆，并进行细胞生长曲线测定、裸鼠皮下及脾包膜下移植试验。结果 转染反义表达载体后，肝癌细胞ｍＲＮＡ和蛋白表达下降，在体内、体外增殖加速；转染正义表达载体后，出现与之相反的结果。正义表达细胞接种组裸鼠肿瘤结节形成

Effect of nm23H1 on proliferation, tumor formation and metastasis of hepatocarcinoma

OBJECTIVE: To study the effects of nm23H1 on in vitro proliferation, in vivo tumor formation and metastasis of hepatocarcinoma. METHODS: Vectors containing insert of either sense or antisense nm23H1 cDNA under the albumin enhancer/promoter for liver cell-specific expression were constructed. The expression vectors were transfected into hepatocarcinoma cell line SMMC-7721. Stable cell clones with maximum and minimum expression of nm23 were obtained. Cell growth was measured in vitro and metastasis was observed in vivo by injecting cells into nude mice, subcutaneously or under the spleen capsule. RESULTS: In vitro, cell clone expressing antisense nm23H1 grew faster than that expressing sense nm23H1. In nude mice, the subcutaneously inoculated tumor grew faster and more aggressively in mice bearing antisense nm23H1 transfected hepatoma. So did metastasis of the antisense nm23H1 transfected hepatoma under the spleen capsule. CONCLUSION: Apart from suppressing metastasis, nm23H1 significantly suppresses tumor cell proliferation in vitro and tumor growth in vivo.