| 弓形虫主要表面抗原基因克隆及在大肠杆菌中的表达 |
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| 引用本文： | 陈慧红,古钦民,卞继峰. 弓形虫主要表面抗原基因克隆及在大肠杆菌中的表达[J]. 山东大学学报(医学版), 1999, 0(2) |
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| 作者姓名： | 陈慧红 古钦民 卞继峰 |
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| 作者单位： | 山东医科大学 |
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| 摘 要： | 为在大肠杆菌中表达弓形虫主要表面抗原（ＳＡＧ１），进一步研究其生物学功能。将ＳＡＧ１基因重组于ｐＧＥＭＥＸ－１融合型表达载体上，转化大肠杆菌ＪＭ１０９（ＤＥ３），得到含重组表达质粒ｐＧＥＭＥＸ－１／ＳＡＧ１的工程菌。结果表明ＩＰＴＧ诱导表达后，经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ－ｂｌｏｔ检测证实含有ＳＡＧ１蛋白。提示ｐＧＥＭＥＸ－１大肠杆菌系统可以表达具有免疫活性的ＳＡＧ１蛋白。
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| 关 键 词： | 弓形虫属；抗原.表面；克隆.分子；大肠杆菌；聚合酶链反应 |
CLONING AND EXPRESSION OF GENE OF THE MAJOR SURFACE ANTIGEN OF TOXOPLASMA GONDII IN ESCHRICHIA COLI |
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| Chen Huihong,Gu Qinmin,Bian Jifeng. CLONING AND EXPRESSION OF GENE OF THE MAJOR SURFACE ANTIGEN OF TOXOPLASMA GONDII IN ESCHRICHIA COLI[J]. Journal of Shandong University:Health Sciences, 1999, 0(2) |
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| Authors: | Chen Huihong Gu Qinmin Bian Jifeng |
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| Affiliation: | Chen Huihong1,Gu Qinmin1,Bian Jifeng2 |
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| Abstract: | To express the major surface antigen of Toxoplasma gondii in Eschrichia. coli for further biological studies. Gene encoding SAG1 of Toxoplasma gondii was cloned into fusion vector pGEMEX1 and transformed into Eschrichia coli JM109 (DE3). With IPTG inducing, SAG1 gene was expressed into fusion protein which was indentified by SDSPAGE and Westenblot analysis . The results revealed that pGEMEX1 Eschrichia coli expression system may be used for expression of SAG1 gene, with immunological activities. |
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| Keywords: | Toxoplasma gondii Antigen surface Clone molecule Eschrichia coli Polymerase chain reaction |
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