动脉内膜损伤及三种反义c-myc RNA的作用

了解反义ｃ－ｍｙｃ基因导入对血管壁的影响。方法将分别载有ｃ－ｍｙｃ第１、２和３外显子反义片段的三种重组逆转录病毒表达载体ａＭ１、ａＭ２和ａＭ３导入球囊损伤的兔股动脉壁，并于术后２１天表达检测。

Arterydenuded model and effects of threekinds of antisensec-myc RNA

OBJECTIVE: To investigate the effects of antisense c-myc on injured vascular wall. METHODS: Three recombinant retroviral expression vectors, aM1, aM2 and aM3, which loading the reverse fragment of exon1, 2 and 3 respectively, were transferred into denuded rabbit femoral artery wall and were assayed 21 days after operation. RESULTS: (1) The regions of rabbit femoral artery were not only convenient for injury control, drug giving and material drawing, but also gave a neointimal area to media area vatio of 1.37:1. Their intensity and area of c-myc expression in media increased to 1.8 and 1.5 fold of normal value. (2) c-myc expression in aM2, aM3 and aM1 was reduced by 37.5%, 20.8%, and 17.8% with media intensity, 71.5%, 57.2%, and 61.6% with media area and 31.3%, 20.8%, and 17.8% with neointimal intensity respectively. (3) Neointimal area/media area of aM2 group reduced by 72.6%, aM3 group reduced by 35.2%, aM1 group had no significant difference comparing with control group. (4) Proliferating cellular nuclear antigen (PCNA) in the intimal and media of the three antisense transferred groups was enhanced with their expressive intensities, but reduced with expressive areas significantly. (5) With the declining of neointimal area/media area, alpha-SM actin expression in the intimal and media of the three antisense transferred groups was significantly increased (aM2 > aM3 > aM1). CONCLUSION: (1) Rabbit femoral artery denuded model is a satisfactory animal model. (2) Antisense c-myc transferring can reduce or resist the c-myc increase in injured artery wall, resist neointimal hyperplasia and smooth muscle cells dedifferentiation,especially c-myc exon 2 antisense RNA vector aM2.