应用实时荧光聚合酶链反应技术与第二代杂交捕获法检测高危亚型人乳头瘤病毒

目的比较实时荧光聚合酶链反应（PCR）与第2代杂交捕获法（HC-Ⅱ）2种人乳头瘤病毒（HPV）检测方法。方法采用实时PCR与HC-Ⅱ分别检测223例宫颈脱落细胞标本中的高危亚型HPV，对结果不一致的标本进行测序分析。结果实时PCR和HC-Ⅱ2种方法检测高危亚型HPV的一致性较好，总符合率为77％，总一致性指数（Kappa指数）为0．538。其中，高度鳞状上皮细胞内病变（HSIL）组二者的符合率为82％，Kappa指数为0．410，一致性比其他组好。结论实时PCR与HC-Ⅱ检测结果一致性较好，尤其对于HSIL患者，可作为临床上HPV高危亚型检测的有效手段。

Application of real-time polymerase chain reaction and hybrid capture 1I in Human papillomavirus detection

Objective To compare real-time polymerase chain reaction （PCR） with hybrid capture Ⅱ （ HC-Ⅱ）for detection of Human papillomavirus （HPV）. Methods Real-time PCR and HC-Ⅱ were used for detection of high-risk HPV in 223 female genital samples. Discordant results between the two methods were confirmed with DNA sequencing. Results The results of real-time PCR and HC-Ⅱ showed good accordance for detection of high-risk HPV in female genital samples. The total accordant rate of real-time PCR and HC-Ⅱwas 77% and general Kappa index was 0. 538. The accordance of the two methods in high-grade squamous intraepithelial lesion （HSIL） group was much better than in other groups. The accordant rate and Kappa index of HSIL group were 82% and 0. 410 respectively. Conclusions Real-time PCR is an effective method for detection of high-risk HPV infection in cervix, and is in good accordance with HC-Ⅱ, especially for HSIL samples.