羟基红花黄色素A对裸鼠人胃腺癌BGC 823移植瘤血管及VEGF，bFGF mRNA表达的影响

目的:研究羟基红花黄色素A(HSYA)对人癌裸鼠皮下移植瘤血管生长、血管内皮生长因子(vascular endothelial growth factor,VEGF)及碱性成纤维生长因子(basic fibroblast growth factor,bFGF)mRNA表达的影响.方法:运用BALB/C nu/nu裸小鼠接种人胃腺癌细胞株BGC-823于右前肢腋部皮下建立裸鼠人癌移植瘤模型,随机分为模型组、阳性药对照组、HSYA高、低剂量组,模型组给予生理盐水腹腔注射,HSYA两组分别给予0.056,0.028 g·L~(-1)HSYA溶液腹腔注射,此3组均每只每日注射2次,间隔4～6 h,每次0.2 mL;阳性药对照组给予2 g·L~(-1)环磷酰胺(cytoxan,CTX)每只腹腔注射0.2 mL,每2天1次.20 d后,检测各组肿瘤体积、重量,光镜观察瘤组织病理改变,实时荧光定量PCR(real time quantitative PCR,RT-qPCR)检测瘤组织VEGF和bFGF mRNA的表达.结果:HSYA 0.028 g·L~(-1)组移植瘤体积(607.42±252.96)mm~3、质量(0.88±0.14)g,VEGF mRNA表达0.49±0.13,bFGF mRNA表达0.60±0.48均较模型组低(P＜0.05或P＜0.01);给药组较模型组瘤组织血管生成明显减少.结论:一定浓度的HSYA有抑制肿瘤生长的作用,其机制可能与抑制肿瘤血管生成有关.

Effects of hydroxy safflor yellow A on blood vessel and mRNA expressionwith VEGF and bFGF of transplantation tumor withgastric adenocarcinoma cell line BGC 823 in nude mice

Objective: To investigate the effects of Hydroxy Safflor yellow A (HSYA) on the growth of blood vessel of transplantation tumor of gastric adenocarcinoma cell line BGC 823 in nude mice and its underlying mechanism of antagonizing tumor angiogenesis. Method: The BGC 823 cells was subcutaneouly injected into the right anterior armpit of BALB/C nu/nu nude mice and established the animal model of transplantation tumor. Then nude mice were divided into 4 groups at random: model group, control group, high or low dosage of HSYA group. The model group were treated with normal sodium by intraperitoneal injection, HSYA groups were treated with HSYA at concentration of 0.056 g·L-1 and 0.028 g·L-1 by intraperitoneal injection, and in these 3 groups each mouse was injected 2 times everyday with 0.2 mL by 4 6 hours interval. The control group were injected in enterocoelia 1 times every 2 days starting from the third day with cytoxan at 2 g·L-1. 20 days later, the volume and weight of nude mice were observed. The pathological change of tumor tissue was observed under optical microscope. The mRNA expression of VEGF and bFGF of transplantation tumor were detected by real time quantitative PCR. Result: The volume(607.42±252.96)mm3 ,  weight (0.88±0.14)g of transplantation tumor, the mRNA expression level of VEGF0.49±0.13 and bFGF 0.60±0.48 are reduced significantly after treatment with HSYA at the concentration of 0.028 g·L-1  compared with physiologic saline treated group(P<0.05 or P<0.01). The tumor pathological angiogenesis of HSYA group is also less obvious than the normal sodium treated group. Conclusion: HSYA in given concentration can inhibit the growth of BGC 823 transplantation tumor, and decreasing the mRNA exprerssion of VEGF and bFGF, which suggests that inhibiting tumor angiogenesis may be one of the mechanisms of HSYA antagonizing tumor.